Lockhart Lesley, Oliver Stephen G, Delneri Daniela
School of Biological Sciences, University of Manchester, 2.205 Stopford Building, Oxford Road, Manchester M13 9PT, UK.
Yeast. 2002 Mar 30;19(5):441-8. doi: 10.1002/yea.852.
In order to investigate the extent of genome rearrangements in laboratory and industrial yeast strains, a set of plasmids, containing ca. 300 bp fragments from highly conserved genes from S. cerevisiae, has been constructed. We chose three unique PCR products, each from a single gene, per chromosome: one from close to the centromere, and one from each chromosome end. Using these plasmids as probes to hybridize a Southern blot from a pulsed-field gel electrophoresis separation of the 16 yeast chromosomes, it is possible to identify large chromosomal rearrangements such as reciprocal translocations.
为了研究实验室和工业酵母菌株中基因组重排的程度，已构建了一组质粒，这些质粒包含来自酿酒酵母高度保守基因的约300 bp片段。我们在每条染色体上选择了三个独特的PCR产物，每个产物来自单个基因：一个来自靠近着丝粒的位置，另一个来自每条染色体的末端。使用这些质粒作为探针，与通过脉冲场凝胶电泳分离16条酵母染色体得到的Southern印迹杂交，就有可能识别出大型染色体重排，如相互易位。
