Sun Feng-Peng, Song Yu-Gang, Cheng Wei, Zhao Tong, Yao Yong-Li
Department of Gastroenterology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, Guangdong Province, China.
World J Gastroenterol. 2002 Apr;8(2):375-8. doi: 10.3748/wjg.v8.i2.375.
To investigate the relationship among gastrin, somatostatin, G and D cells in gastric ulcer and in its healing process in rats.
Fourty-nine Wistar rats were divided into 7 groups. The gastric ulcer model was induced by acetic acid successfully. The gastrin and the somatostatin in rat plasma, gastric fluid and antral tissue were measured by radioimmunoassay(RIA). G and D cells in antral mucosa were analyzed with polyclonal antibody of gastrin and somatostatin by immunohistochemical method and Quantimet 500 image analysis system.
In gastric ulcer, the level of gastrin in plasma, gastric fluid, and antral tissue increased, that of somatostatin declined, and the disorder gradually recovered to the normal level in the healing process. Immunohistochemical technique of G and D cells in antral mucosa demonstrated that the number of G cells increased and that of D cells decreased, both areas of G and D cells declined, the ratio of number and area of G/D increased in gastric ulcer, and the disorder gradually recovered in the healing process.
In gastric ulcer, the increased gastrin secreted by G cells, the declined somatostatin secreted by D cells, and the disordered G/D cell ratio can lead to gastrointestinal dysfunction.
探讨大鼠胃溃疡及其愈合过程中胃泌素、生长抑素、G细胞和D细胞之间的关系。
将49只Wistar大鼠分为7组。用乙酸成功诱导出胃溃疡模型。采用放射免疫分析法（RIA）测定大鼠血浆、胃液和胃窦组织中的胃泌素和生长抑素。用胃泌素和生长抑素的多克隆抗体通过免疫组织化学方法和Quantimet 500图像分析系统分析胃窦黏膜中的G细胞和D细胞。
胃溃疡时，血浆、胃液和胃窦组织中的胃泌素水平升高，生长抑素水平下降，且在愈合过程中这种紊乱逐渐恢复至正常水平。胃窦黏膜G细胞和D细胞的免疫组织化学技术显示，胃溃疡时G细胞数量增加，D细胞数量减少，G细胞和D细胞的面积均减小，G/D细胞数量与面积之比增加，且在愈合过程中这种紊乱逐渐恢复。
在胃溃疡中，G细胞分泌的胃泌素增加，D细胞分泌的生长抑素减少，以及G/D细胞比例失调可导致胃肠功能紊乱。
