十五肽BPC 157对大鼠胃溃疡的保护作用
Protective effects of pentadecapeptide BPC 157 on gastric ulcer in rats.
作者信息
Xue Xiao-Chang, Wu Yong-Jie, Gao Ming-Tang, Li Wen-Guang, Zhao Ning, Wang Zeng-Lu, Bao Chun-Jie, Yan Zhen, Zhang Ying-Qi
机构信息
Biotechnology Centre, Fourth Military Medical University, 169 West Changle Road, Xi'an 710032, Shaanxi Province, China.
出版信息
World J Gastroenterol. 2004 Apr 1;10(7):1032-6. doi: 10.3748/wjg.v10.i7.1032.
AIM
To investigate the protective effects of gastric pentadecapeptide BPC 157 on acute and chronic gastric ulcers in rats and to compare the results in therapy of human gastric ulcers by different administration methods.
METHODS
Gastric pentadecapeptide BPC 157 was administered (initial single or continuous administration) into rats either intragastrically or intramuscularly before (induced acute gastric ulcer) or after (induced chronic gastric ulcer) the applications of inducing agents, and each animal was sacrificed to observe the protective effects of BPC 157 on gastric ulcers.
RESULTS
Both intramuscular (im) and intragastric (ig) administration of BPC 157 could apparently reduce the ulcer area and accelerate the healing of induced ulcer in different models and the effect of im administered BPC 157 was better than that of ig. The rats treated with higher dosages (400 ng/kg, 800 ng/kg) of BPC 157 (im and ig) showed significantly less lesion (P<0.01 vs excipient or saline control), the inhibition ratio of ulcer formation varied between 45.7% and 65.6%, from all measurements except 400 ng/kg BPC 157 in pylorus ligation induced model (P<0.05), in which the inhibition rate was 54.2%. When im administered (800 ng/kg BPC 157) in three models, the inhibition ratio of ulcer formation was 65.5%, 65.6% and 59.9%, respectively, which was better than that of famotidine (its inhibition rate was 60.8%, 57.2% and 34.3%, respectively). Continuous application of BPC 157 (in chronic acetate induced gastric ulcer) could accelerate rebuilding of glandular epithelium and formation of granulation tissue (P<0.05 at 200 ng/kg and P<0.01 at 400 ng/kg and 800 ng/kg vs excipient or saline control).
CONCLUSION
Both im and ig administered gastric pentadecapeptide BPC 157 can apparently ameliorate acute gastric ulcer in rats and antagonize the protracted effect of acetate challenge on chronic ulcer. The effect of im administration of BPC 157 is better than that of ig, and the effective dosage of the former is lower than that of the latter.
目的
研究胃十五肽BPC 157对大鼠急性和慢性胃溃疡的保护作用，并比较不同给药方法治疗人类胃溃疡的效果。
方法
在应用诱导剂之前（诱导急性胃溃疡）或之后（诱导慢性胃溃疡），将胃十五肽BPC 157经胃内或肌肉注射给予大鼠（初始单次或连续给药），然后处死每只动物，观察BPC 157对胃溃疡的保护作用。
结果
在不同模型中，肌肉注射（im）和胃内注射（ig）BPC 157均能明显减小溃疡面积，加速诱导溃疡的愈合，且肌肉注射BPC 157的效果优于胃内注射。用较高剂量（400 ng/kg、800 ng/kg）的BPC 157（肌肉注射和胃内注射）处理的大鼠病变明显较少（与赋形剂或生理盐水对照组相比，P<0.01），除幽门结扎诱导模型中400 ng/kg BPC 157外（P<0.05），溃疡形成抑制率在45.7%至65.6%之间，其中该组抑制率为54.2%。在三种模型中肌肉注射（800 ng/kg BPC 157）时，溃疡形成抑制率分别为65.5%、65.6%和59.9%，优于法莫替丁（其抑制率分别为60.8%、57.2%和34.3%）。连续应用BPC 157（在慢性乙酸诱导的胃溃疡中）可加速腺上皮的重建和肉芽组织的形成（200 ng/kg时P<0.05，400 ng/kg和800 ng/kg时P<0.01，与赋形剂或生理盐水对照组相比）。
结论
肌肉注射和胃内注射胃十五肽BPC 157均能明显改善大鼠急性胃溃疡，并拮抗乙酸刺激对慢性溃疡的持久影响。肌肉注射BPC 157的效果优于胃内注射，且前者的有效剂量低于后者。
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