Nagamine M, Okuma S
Clin Chim Acta. 1975 Nov 15;65(1):39-46. doi: 10.1016/0009-8981(75)90332-0.
An unusual isoenzyme pattern of serum alkaline phosphatase was found in a patient with skeletal abnormalities due to multiple epiphyseal dysplasia and it was demonstrated that the abnormal pattern was caused by complex formation between serum alkaline phosphatase and immunoglobulin G of the lambda class. Physicochemical studies of the patient's serum alkaline phosphatase showed the properties of an osseous enzyme. Evidence was obtained indicating that the complexing occurred in vitro and that the patient's immunoglobulin G had the ability to bind the hepatic and osseous isoenzymes selectively but not to bile, placental and intestinal isoenzymes. No abnormality was detected in the leucocyte isoenzyme pattern. The relationship between the occurrence of complex formation and the patient's bone disease was not established.
在一名因多发性骨骺发育异常而骨骼异常的患者中发现了一种不寻常的血清碱性磷酸酶同工酶模式，结果表明这种异常模式是由血清碱性磷酸酶与λ类免疫球蛋白G之间形成复合物所致。对该患者血清碱性磷酸酶的物理化学研究显示出一种骨酶的特性。有证据表明这种复合作用在体外发生，并且该患者的免疫球蛋白G有能力选择性地结合肝脏和骨同工酶，但不结合胆汁、胎盘和肠道同工酶。在白细胞同工酶模式中未检测到异常。复合物形成的发生与患者骨病之间的关系尚未明确。
