Mäder M, Kolbus N, Meihorst D, Köhn A, Beuche W, Felgenhauer K
Neurologische Klinik der Universität, Göttingen, Germany.
Clin Exp Immunol. 1994 Jan;95(1):98-102. doi: 10.1111/j.1365-2249.1994.tb06021.x.
Patterns of alkaline phosphatase (AP)-binding proteins were observed in the alkaline pH range of 6.5-9.5 upon isoelectric focusing and blotting of serum from patients with inflammatory diseases. After isolation using affinity chromatography on protein A or immunoaffinity chromatography on AP coupled to cyanogen bromide (CNBr)-activated Sepharose, the AP-binding protein was identified as IgG on Western blots and in ELISA using human IgG-specific antibodies. It was shown that this IgG binds to AP from both calf (bovine) and human intestine. However, it binds neither to the human liver-bone-kidney (LBK) isoform nor to bacterial AP. Moderate reaction was observed with human placental AP. Comparing patients with various diagnoses (n = 284), AP-binding antibodies were mainly found in severe bacterial infections. They were not detected in serum from healthy blood donors (n = 300). The presence of AP-binding IgG was independent of the infected organ and the bacterial species causing infection. This antibody may be useful for discriminating bacterial from viral infection and for indicating severe bacterial inflammation.
在对炎症性疾病患者的血清进行等电聚焦和印迹分析时，在6.5至9.5的碱性pH范围内观察到碱性磷酸酶（AP）结合蛋白的模式。使用蛋白A亲和色谱或与溴化氰（CNBr）活化的琼脂糖偶联的AP进行免疫亲和色谱分离后，在蛋白质印迹和使用人IgG特异性抗体的ELISA中，AP结合蛋白被鉴定为IgG。结果表明，这种IgG可与来自小牛（牛）和人肠道的AP结合。然而，它既不与人肝-骨-肾（LBK）同工型结合，也不与细菌AP结合。与人胎盘AP有中等反应。比较不同诊断的患者（n = 284），AP结合抗体主要存在于严重细菌感染中。在健康献血者的血清中未检测到（n = 300）。AP结合IgG的存在与感染器官和引起感染的细菌种类无关。这种抗体可能有助于区分细菌感染和病毒感染，并指示严重的细菌炎症。
