de Souza C P, Pereira J P, de Azevedo M de L, Mendes N M, Paulini E
Rev Bras Pesqui Med Biol. 1979 Sep;12(4-5):265-71.
The influence of EDTA on B. glabrata has been investigated. Newly hatched snails were exposed to concentrations of EDTA from 1 to 64 ppm, and young (diameter of 6 mm) snails to concentrations of 50, 80 and 100 ppm, for periods of 90 days. Fifty percent reduction of egg productivity has been caused by concentration of 16 ppm of EDTA while 50% of mortality has occurred at about 70 ppm. The calcium and iron content both in treated and non-treated young snails have been estimated by atomic absorption photometry. The uptake of calcium was 40, 83 and 90% less for calcium and 37, 77 and 81% less for iron as compared with the untreated group. The calcium content of the shell was 5--15 times greater than that of the soft body, while the iron content of those two parts was in the proportion of 1:1. These proportions were maintained constant in the treated and non-treated groups. The interference of increasing concentrations of EDTA has resulted in the proportional reduction of growth-rate, reproduction rate and of longevity of the exposed snails.
已对乙二胺四乙酸(EDTA)对光滑双脐螺的影响进行了研究。将刚孵化出的蜗牛暴露于浓度为1至64 ppm的EDTA中,将幼体(直径6毫米)蜗牛暴露于浓度为50、80和100 ppm的EDTA中,持续90天。16 ppm的EDTA浓度导致产卵量减少50%,而在约70 ppm时出现了50%的死亡率。通过原子吸收光度法估算了经处理和未处理的幼体蜗牛中的钙和铁含量。与未处理组相比,处理组蜗牛对钙的摄取量减少了40%、83%和90%,对铁的摄取量减少了37%、77%和81%。贝壳中的钙含量比软体部分高5至15倍,而这两部分的铁含量比例为1:1。在处理组和未处理组中,这些比例保持恒定。EDTA浓度增加所产生的干扰导致暴露蜗牛的生长速率、繁殖率和寿命成比例降低。