Fosse T, Madinier I, Hannoun L, Giraud-Morin C, Hitzig C, Charbit Y, Ourang S
Laboratoire de Bactériologie, Hôpital l'Archet 2, Centre Hospitalier Universitaire de Nice, Nice, France.
Oral Microbiol Immunol. 2002 Apr;17(2):85-8. doi: 10.1046/j.0902-0055.2001.00096.x.
This prospective study was designed to investigate amoxicillin-resistant oral anaerobes, and to identify their beta-lactamase-encoding genes. Three subgingival bacterial samples were collected from 12 patients suffering from periodontitis. One to seven beta-lactamase-producing strains were obtained from each patient, mostly belonging to the Prevotella genus (Bacteroides eggerthii, 2/35 strains; Prevotella sp., 33/35 strains). PCR assays were used to detect cfxA and cepA/cblA, the genes encoding class A/group2e beta-lactamases previously described in the Bacteroides fragilis group. The present investigation confirmed the role of Prevotella species as beta-lactamase producers in periodontal pockets. Additionally, this PCR screening showed (1): the high prevalence of CfxA beta-lactamase production by aminopenicillin-resistant Prevotella (32/33: 97.0% positive strains) vs. cepA/cblA (1/33: 3.0% positive strains), and (2) the presence of cfxA in the periodontal reservoir in the absence of antimicrobial therapy during the previous 6 months.
本前瞻性研究旨在调查耐阿莫西林的口腔厌氧菌，并鉴定其β-内酰胺酶编码基因。从12名牙周炎患者中采集了三份龈下细菌样本。从每位患者中获得1至7株产β-内酰胺酶的菌株，大多数属于普雷沃菌属（埃氏拟杆菌，2/35株；普雷沃菌属，33/35株）。采用聚合酶链反应（PCR）检测cfxA和cepA/cblA，这两个基因编码先前在脆弱拟杆菌群中描述的A类/2e组β-内酰胺酶。本研究证实了普雷沃菌属作为牙周袋中产β-内酰胺酶菌的作用。此外，该PCR筛选显示：（1）耐氨基青霉素的普雷沃菌产生CfxAβ-内酰胺酶的比例很高（32/33：97.0%阳性菌株），而cepA/cblA的比例较低（1/33：3.0%阳性菌株）；（2）在过去6个月未进行抗菌治疗的情况下，牙周菌库中存在cfxA。
