[1-14C]辛酸在大鼠脑片中的体外摄取：评估[1-11C]辛酸作为神经胶质功能PET示踪剂的基础研究

In vitro uptake of [1-14C]Octanoate in brain slices of rats: basic studies for assessing [1-11C]Octanoate as a PET tracer of glial functions.

作者信息

Kuge Yuji, Hikosaka Kenji, Seki Koh-ichi, Ohkura Kazue, Nishijima Ken-ichi, Tsukamoto Eriko, Tamaki Nagara

机构信息

Department of Tracer Kinetics, Graduate School of Medicine, Hokkaido University, Kita 15 Nishi 7, Kita-ku, 060-8638, Sapporo, Japan.

出版信息

Nucl Med Biol. 2002 Apr;29(3):303-6. doi: 10.1016/s0969-8051(01)00309-2.

DOI:10.1016/s0969-8051(01)00309-2

PMID:11929699

Abstract

To clarify the contribution of glial cells to octanoate uptake into the brain, we determined the effects of fluoroacetate, a selective inhibitor of glial metabolism, on in vitro brain uptake of [1-14C]octanoate, using rat brain slices. The [1-14C]octanoate uptake significantly decreased, depending on the concentration of fluoroacetate (p = 0.001). The [1-14C]octanoate uptakes at 5 mM (0.23 +/- 0.05% uptake/mg slice) and 25 mM fluoroacetate (0.12 +/- 0.01% uptake/mg slice) were significantly lower than that at control (0.29 +/- 0.02% uptake/mg slice, p < 0.05 and p < 0.001, respectively). The results demonstrate the contribution of glial cells to octanoate uptake into the brain. The potential of [1-11C]octanoate as a PET tracer for studying glial functions is suggested.

摘要

为了阐明神经胶质细胞对辛酸摄取进入大脑的作用，我们使用大鼠脑片，测定了神经胶质代谢的选择性抑制剂氟乙酸对[1-14C]辛酸体外脑摄取的影响。[1-14C]辛酸摄取量显著降低，这取决于氟乙酸的浓度（p = 0.001）。5 mM（0.23±0.05%摄取量/毫克脑片）和25 mM氟乙酸时的[1-14C]辛酸摄取量显著低于对照组（0.29±0.02%摄取量/毫克脑片，分别为p < 0.05和p < 0.001）。结果证明了神经胶质细胞对辛酸摄取进入大脑的作用。提示了[1-11C]辛酸作为研究神经胶质功能的正电子发射断层显像（PET）示踪剂的潜力。