酿酒酵母Fin1蛋白在纺锤极体之间形成细胞周期特异性细丝。
The Saccharomyces cerevisiae Fin1 protein forms cell cycle-specific filaments between spindle pole bodies.
作者信息
van Hemert Martijn J, Lamers Gerda E M, Klein Dionne C G, Oosterkamp Tjerk H, Steensma H Yde, van Heusden G Paul H
机构信息
Section Yeast Genetics, Institute of Molecular Plant Sciences, Leiden University, Wassenaarseweg 64, 2333AL, Leiden, The Netherlands.
出版信息
Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5390-3. doi: 10.1073/pnas.072556099. Epub 2002 Apr 2.
The FIN1 gene from the yeast Saccharomyces cerevisiae encodes a basic protein with putative coiled-coil regions. Here we show that in large-budded cells a green fluorescent protein-Fin1 fusion protein is visible as a filament between the two spindle pole bodies. In resting cells the protein is undetectable, and in small-budded cells it is localized in the nucleus. During late mitosis it localizes on the spindle pole bodies. Filaments of cyano fluorescent protein-tagged Fin1 colocalize with filaments of green fluorescent protein-tagged Tub1 only in large-budded cells. By electron and atomic force microscopy we showed that purified recombinant Fin1p self-assembles into filaments with a diameter of approximately 10 nm. Our results indicate that the Fin1 protein forms a cell cycle-specific filament, additional to the microtubules, between the spindle pole bodies of dividing yeast cells.
来自酿酒酵母的FIN1基因编码一种具有假定卷曲螺旋区域的碱性蛋白。我们在此表明，在大芽殖细胞中，绿色荧光蛋白-Fin1融合蛋白在两个纺锤体极体之间呈现为细丝状。在静止细胞中无法检测到该蛋白，而在小芽殖细胞中它定位于细胞核。在有丝分裂后期，它定位于纺锤体极体。仅在大芽殖细胞中，氰基荧光蛋白标记的Fin1细丝与绿色荧光蛋白标记的Tub1细丝共定位。通过电子显微镜和原子力显微镜，我们表明纯化的重组Fin1p自组装成直径约为10 nm的细丝。我们的结果表明，在分裂酵母细胞的纺锤体极体之间，Fin1蛋白形成了一种除微管之外的细胞周期特异性细丝。
Zotero 插件