Yan Ming, Zhang Li-Cai, Dai Ti-Jun, Zheng Yin-Ming, Duan Shi-Ming
Department of Anesthesiology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006;
Sheng Li Xue Bao. 2002 Feb 25;54(1):60-4.
In formalin pain model, the effect of propofol on Fos expression in the spinal cord was examined by means of c -fos oncogene immunohistochemistry and NADPH-d histochemistry. Fos-like immunoreactive (FLI) neurons were mainly found in the ipsilateral dorsal horn after injection of formalin, some of which were FLI/NOS double-labeled neurons. Most of the FLI or FLI/NOS double-labeled neurons were observed in the medial part of lamina and the outer lamina . Before or after injection of formain, i.p. injection of propofol significantly decreased the number of FLI and FLI/NOS double-labeled neurons in all laminae (P<0.05 or P<0.01). By single i.p. injection of propofol or normal saline, few FLI neurons were found in the spinal cord. The results suggest that the antinociceptive function of propofol is possibly involved in the depression of the NOS neurons in the spinal cord.
在福尔马林疼痛模型中，通过c-fos癌基因免疫组织化学和NADPH-d组织化学方法检测丙泊酚对脊髓中Fos表达的影响。注射福尔马林后，Fos样免疫反应性（FLI）神经元主要出现在同侧背角，其中一些是FLI/NOS双标记神经元。大多数FLI或FLI/NOS双标记神经元见于板层内侧和外层。在注射福尔马林之前或之后，腹腔注射丙泊酚显著减少了所有板层中FLI和FLI/NOS双标记神经元的数量（P<0.05或P<0.01）。通过单次腹腔注射丙泊酚或生理盐水，在脊髓中发现的FLI神经元很少。结果表明，丙泊酚的镇痛功能可能与脊髓中NOS神经元的抑制有关。
