Cao J L, Zeng Y M, Zhang L C, Duan S M
Department of Anesthesiology, Affiliated Hospital of Xuzhou Medical College, China.
Sheng Li Xue Bao. 2000 Jun;52(3):235-8.
Fos immunocytochemistry, NADPH-d histochemistry and Fos/NADPH-d double-labeling method were used to study the changes in formalin-induced Fos, NADPH-d positive and Fos/NADPH-d double-labeled neurons in the spinal cord of morphine-tolerant rats. Formalin-induced Fos-like immunoreactivity (Fos-LI) was located in the superficial laminae and neck of ipsilateral spinal cord. Acute administration of morphine decreased the expression of Fos-LI in non-tolerant rats, while the expression of Fos-LI was significantly increased in morphine-tolerant rats. Fos-LI was distributed not only in the whole laminae of the ipsilateral spinal cord but also in the contralateral spinal cord. Acute administration of morphine was ineffective in decreasing the expression of Fos-LI in morphine-tolerant rats. Morphine tolerance increased the expression of formalin-induced NADPH-d positive neurons in the superficial laminae of spinal dorsal horn. A few formalin-induced Fos/NADPH-d double-labeled neurons were detected in the superficial laminae of spinal dorsal horn of non-tolerant rats. In morphine-tolerant rats, on the other hand, formalin-induced Fos/NADPH-d double-labeled neurons were increased and distributed in the whole laminae of the ipsilateral spinal cord and the contralateral superficial spinal cord. It is suggested that NO is involved in the increase of formalin-induced Fos-LI in the spinal cord of morphine-tolerant rats and may play an important role in the development of morphine tolerance.
采用Fos免疫细胞化学、NADPH-d组织化学及Fos/NADPH-d双标记法，研究吗啡耐受大鼠脊髓中福尔马林诱导的Fos、NADPH-d阳性及Fos/NADPH-d双标记神经元的变化。福尔马林诱导的Fos样免疫反应性（Fos-LI）位于同侧脊髓的浅层和颈部。急性给予吗啡可降低非耐受大鼠Fos-LI的表达，而在吗啡耐受大鼠中Fos-LI的表达显著增加。Fos-LI不仅分布于同侧脊髓的全层，也分布于对侧脊髓。急性给予吗啡对降低吗啡耐受大鼠Fos-LI的表达无效。吗啡耐受增加了脊髓背角浅层福尔马林诱导的NADPH-d阳性神经元的表达。在非耐受大鼠的脊髓背角浅层检测到少量福尔马林诱导的Fos/NADPH-d双标记神经元。另一方面，在吗啡耐受大鼠中，福尔马林诱导的Fos/NADPH-d双标记神经元增加，并分布于同侧脊髓的全层和对侧脊髓浅层。提示一氧化氮参与了吗啡耐受大鼠脊髓中福尔马林诱导的Fos-LI增加，可能在吗啡耐受的形成中起重要作用。
