Almeida A Paulo G, Billingsley Peter F
Medical Entomology Unit, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Portugal.
J Med Entomol. 2002 Jan;39(1):207-14. doi: 10.1603/0022-2585-39.1.207.
Two subeellular fractions from the midgut of the malaria mosquito Anopheles stephensi (Liston) were used to immunize BALB/c mice. Mice were subsequently infected with the rodent malaria parasite Plasmodium berghei (Vineke & Lips), and the effects of anti-mosquito immunity on mosquito survival and fecundity and on parasite transmission were investigated. Mosquitoes were infected directly from mice (in vivo) or by feeding cultured ookinetes through a membrane (in vitro). Infections were monitored by counting oocysts on the midgut wall. Microvilli extracts induced a strong and partially specific antibody reaction against the midgut, which was manifest as decreased survival in in vivo fed mosquitoes and reduced fecundity in both kinds of feeding. Antisera against microvilli reduced the mean intensity of P. berghei oocysts when fed in vitro, while mosquitoes fed antiserum against basolateral plasma membranes in vivo, showed higher oocyst burdens.
利用来自疟疾蚊子斯氏按蚊（利斯顿）中肠的两个亚细胞部分免疫BALB/c小鼠。随后用啮齿类疟原虫伯氏疟原虫（维内克和利普斯）感染小鼠，并研究抗蚊子免疫对蚊子存活、繁殖力以及寄生虫传播的影响。蚊子通过直接从感染的小鼠获取（体内感染）或通过膜饲以培养的动合子（体外感染）。通过计数中肠壁上的卵囊来监测感染情况。微绒毛提取物诱导产生了针对中肠的强烈且部分特异性的抗体反应，这表现为体内饲血的蚊子存活率降低以及两种饲血方式下的繁殖力下降。体外饲血时，抗微绒毛抗血清降低了伯氏疟原虫卵囊的平均强度，而体内饲以抗基底外侧质膜抗血清的蚊子显示出更高的卵囊负荷。
