Kocazeybek Bekir, Arabaci Umit, Akdur Hülya, Sezgiç Metin, Erentürk Selim
Microbiology and Clinical Microbiology Department, University of Istanbul, Cerrahpaşa Medical Faculty, Turkey.
Transfus Apher Sci. 2002 Feb;26(1):29-34. doi: 10.1016/s1473-0502(01)00141-0.
Platelets, which have an important role in hemostatic mechanisms and which were prepared by single and random methods were investigated to measure quantity, and aggregation response during 5 days of storage. The aggregation response and quantitative values of platelet concentrates (PCs), 60 of which were prepared by a single donor method and 62 by a random method were investigated during the 1st, 3rd, and 5th days of storage. The single donor platelets (SDP) were obtained by using the MCS Plus apheresis device and the random donor platelets (RDP) were obtained by two-phase centrifugation in the Heraeus 8500i centrifuge device (during the first phase, platelet rich plasma was obtained then platelet concentrate was obtained from this product) and were stored at 22 degrees C on a circular agitator. In addition, pH, PO2, PCO2, glucose and lactate values were measured in order to evaluate the effects of storage. The aggregation response was measured using adenosine diphosphate (ADP), epinephrine (EPN), collagen (COLL) and ristocetin (RIST). The cell count in mm3 and the total cell count were also measured. The total cell counts and cells in mm3 of the PCs which were prepared by the single donar method on the 1st, 3rd and 5th days, were: 3.11 x 10(11), 3.09 x 10(11), 3.07 x 10(11) and 292 x 10(3), 290 x 10(3), 289 x 10(3) and of those prepared by the random method were: 5.71 x 10(10), 5.69 x 10(10), 5.66 x 10(10) and 156 x 10(3), 153 x 10(3), 151 x 10(3). The mean aggregation responses of the PCs prepared by the two methods on the 1st, 3rd and 5th days, expressed as a % were: ADP: 94.8-93.2, 81.6-78.7, 44.3-8.2; COLL: 91.7-89.6, 79.2-74.2, 29.8-11.1; EPN: 88.5-91.3, 64.2-62.7, 39.4-4.5 and RIST: 89.4-89.4, 76.5-73.6, 14.4-3.2. Other data related to platelet storage were obtained by measuring the pH, PO2, PCO2, glucose and lactate levels of the PCs. In our study, it was determined that in spite of the optimal storage conditions, the aggregation response of the PCs decreased significantly, whereas, the numerical values changed little during the storage period.
对在止血机制中起重要作用且通过单一和随机方法制备的血小板进行了研究，以测量其在储存5天期间的数量和聚集反应。在储存的第1天、第3天和第5天，对60份通过单一供体方法制备和62份通过随机方法制备的血小板浓缩物（PCs）的聚集反应和定量值进行了研究。单一供体血小板（SDP）通过使用MCS Plus血液成分分离设备获得，随机供体血小板（RDP）通过在Heraeus 8500i离心机设备中进行两相离心获得（在第一阶段，获得富血小板血浆，然后从该产品中获得血小板浓缩物），并在22摄氏度下于圆形搅拌器上储存。此外，为了评估储存的影响，还测量了pH、PO2、PCO2、葡萄糖和乳酸值。使用二磷酸腺苷（ADP）、肾上腺素（EPN）、胶原蛋白（COLL）和瑞斯托菌素（RIST）测量聚集反应。还测量了每立方毫米的细胞计数和总细胞计数。通过单一供体方法在第1天、第3天和第5天制备的PCs的总细胞计数和每立方毫米的细胞数分别为：3.11×10¹¹、3.09×10¹¹、3.07×10¹¹和292×10³、290×10³、289×10³，通过随机方法制备的分别为：5.71×10¹⁰、5.69×10¹⁰、5.66×10¹⁰和156×10³、153×10³、151×10³。两种方法在第1天、第3天和第5天制备的PCs的平均聚集反应以百分比表示为：ADP：94.8 - 93.²、81.6 - 78.7、44.3 - 8.2；COLL：91.7 - 89.6、79.2 - 74.2、29.8 - 11.1；EPN：88.5 - 91.3、64.2 - 62.7、39.4 - 4.5；RIST：89.4 - 89.4、76.5 - 73.6、14.4 - 3.2。通过测量PCs的pH、PO2、PCO2、葡萄糖和乳酸水平获得了与血小板储存相关的其他数据。在我们的研究中，确定尽管储存条件最佳，但PCs的聚集反应显著降低，而在储存期间数值变化很小。
