Glaser A, Friedlein H, Zingsem J, Zimmermann R, Weisbach V, Ruf A, Eckstein R
Department of Transfusion Medicine and Haemostaseology, University Erlangen-Nürnberg, Erlangen, Germany.
J Clin Apher. 2001;16(3):148-54. doi: 10.1002/jca.1028.
Modern cell separators allow the collection of two plateletpheresis concentrates (PCs) at one session. This study evaluates the quality of PCs stored as double concentrates in standard storage containers of two manufacturers. We collected 20 PCs that contained 4.5 x 10(11) platelets in 375 ml plasma (10 using the COBE Spectra and 10 using the Fresenius AS.TEC 204 with 500 ml bags) that were split into one unit of 3.0 x 10(11) platelets in 250 ml (3.0-PC) and one of 1.5 x 10(11) platelets in 125 ml (1.5-PC). Storage of one 3.0-PC per bag of a two-bag system corresponded to storage conditions for double PCs and storage of one 1.5-PC per bag to storage conditions of single PCs. Cell counts, blood gas analysis, glucose and lactate levels, platelet aggregation, and activation and plasma levels of beta- thromboglobulin (beta-TG) and complement factor 3a (C3a) were measured before storage and again on days 3 and 5. COBE 3.0-PCs demonstrated less pH rise, lactate production, CD 62P expression and beta-TG plasma levels, and better aggregability after storage than COBE 1.5-PCs. Fresenius 1.5-PCs had similar platelet quality to COBE 3.0-PCs. Fresenius 3.0-PCs showed a fall of pH (day 5: 6.22 +/- 0.56), the highest amount of anaerobic glycolysis compared to all other storage conditions investigated, high CD 62P- expression and beta-TG plasma levels, and impaired aggregability on days 3 and 5. The highest C3a levels were found in COBE 1.5-PCs. 3.0 x 10(11) platelets in 250 ml plasma should be stored either in one bag of the COBE system or in two 500 ml bags of the Fresenius system. The COBE two-bag system allows the storage of two PCs without loss of platelet quality. Two PCs should not be stored in the Fresenius C4L 500 ml storage containers.
现代血细胞分离机能够在一次操作中采集两份血小板单采浓缩物（PC）。本研究评估了在两家制造商的标准储存容器中作为双倍浓缩物储存的PC的质量。我们采集了20份PC，每份PC在375毫升血浆中含有4.5×10¹¹个血小板（其中10份使用COBE Spectra，10份使用配有500毫升袋子的费森尤斯AS.TEC 204），将每份PC分成一份在250毫升中含有3.0×10¹¹个血小板的单位（3.0-PC）和一份在125毫升中含有1.5×10¹¹个血小板的单位（1.5-PC）。两袋系统中每袋储存一份3.0-PC对应于双倍PC的储存条件，每袋储存一份1.5-PC对应于单倍PC的储存条件。在储存前以及第3天和第5天再次测量细胞计数、血气分析、葡萄糖和乳酸水平、血小板聚集以及β-血小板球蛋白（β-TG）和补体因子3a（C3a）的活化及血浆水平。与COBE 1.5-PC相比，COBE 3.0-PC在储存后表现出pH升高幅度更小、乳酸生成更少、CD 62P表达和β-TG血浆水平更低以及聚集性更好。费森尤斯1.5-PC的血小板质量与COBE 3.0-PC相似。费森尤斯3.0-PC在第5天pH下降（6.22±0.56），与所有其他研究的储存条件相比厌氧糖酵解量最高，CD 62P表达和β-TG血浆水平高，且在第3天和第5天聚集性受损。在COBE 1.5-PC中发现C3a水平最高。250毫升血浆中3.0×10¹¹个血小板应储存在COBE系统的一个袋子中或费森尤斯系统的两个500毫升袋子中。COBE两袋系统允许储存两份PC且不损失血小板质量。两份PC不应储存在费森尤斯C4L 500毫升储存容器中。
