Seghatchian J, Krailadsiri P, Beard M, Bukasa A, Bashir S
National Blood Service, London & SE, Colindale, UK.
Transfus Apher Sci. 2002 Feb;26(1):47-60. doi: 10.1016/s1473-0502(01)00145-8.
The causes of leucodepletion failure are multifactoral and can be related to haematological variability in blood donors or donation, defective filters, poor specimen handling or ageing, and/or the presence of non-adhering leucocyte/platelets. Since refiltering removes all types of leucocytes, including the populations appearing as extra gated events, we have developed a practical method for refiltering the failed leucodepleted components on standard filters and back-flushing the second filter to assess the nature of the WBC sub-population. In practice, recovered leucocytes from red cell filters and whole blood mainly consist of neutrophils. Those from platelet and plasma filters were mainly lymphocyte with considerable differences depending on the type of leucodepletion process. Atypical leucocytes are often seen in some pre-/post-cellular leucofiltered components. These appear characteristically as small WBC with a lower affinity for filter matrix, or as cell fragment, pinched leucocyte or apoptotic cells. Different reagents in use show variable sensitivity in identifying these extra gatal events. Storage of leucodepleted samples also induces different types of abnormality in leucocyte dot plot. A useful practical approach for characterisation of the nature of leucocyte sub-populations causing failure in leucodepleted components is provided.
白细胞去除失败的原因是多方面的，可能与献血者或献血过程中的血液学变异性、过滤器缺陷、样本处理不当或老化以及/或者存在非粘附性白细胞/血小板有关。由于重新过滤可以去除所有类型的白细胞，包括那些表现为额外门控事件的细胞群体，我们开发了一种实用方法，用于对在标准过滤器上白细胞去除失败的成分进行重新过滤，并对第二个过滤器进行反冲洗，以评估白细胞亚群的性质。实际上，从红细胞过滤器和全血中回收的白细胞主要是中性粒细胞。来自血小板和血浆过滤器的白细胞主要是淋巴细胞，根据白细胞去除过程的类型存在相当大的差异。在一些细胞前/后白细胞过滤成分中经常可以看到非典型白细胞。这些细胞通常表现为对过滤器基质亲和力较低的小白细胞，或者表现为细胞碎片、压缩白细胞或凋亡细胞。使用的不同试剂在识别这些额外门控事件时显示出不同的敏感性。白细胞去除样本的储存也会在白细胞点图中诱导不同类型的异常。本文提供了一种有用的实用方法，用于表征导致白细胞去除成分失败的白细胞亚群的性质。
