Seghatchian Jerard, Krailadsiri Pranee
National Blood Service, London & South East, Colindale, UK.
Transfus Apher Sci. 2002 Feb;26(1):61-72. doi: 10.1016/s1473-0502(01)00146-x.
Characterisation of the nature of leucodepletion failure, based on the analysis of leucocyte sub-populations, is an essential task for continual improvement in the clinical safety of blood components and for the selection of a safe and appropriate leucodepletion process. Such information is also critical for improving the quality of filters through an understanding of the mechanism of leucocyte removal filters. Unfortunately, the residual leucocytes of filtered blood components are around the sensitivity of current leucocyte-counting technologies, hence a need for an enrichment process. This preliminary report deals with the merits of various WBC enrichment strategies for the analysis of WBC sub-population subsets. A new procedure, based on refiltering and backflushing the content, with up to 30-fold enrichment, is described. This one-step procedure has the advantage of concentrating both native and atypical leucocytes seen in blood components. The latter may account for lack of interchangeability of results obtained by various counting methods.
基于白细胞亚群分析来表征白细胞去除失败的性质，对于持续提高血液成分的临床安全性以及选择安全合适的白细胞去除工艺而言是一项重要任务。通过了解白细胞去除过滤器的机制，此类信息对于提高过滤器质量也至关重要。不幸的是，过滤后的血液成分中的残留白细胞处于当前白细胞计数技术的灵敏度附近，因此需要进行富集过程。本初步报告探讨了用于分析白细胞亚群子集的各种白细胞富集策略的优点。描述了一种基于对内容物进行再过滤和反冲洗的新程序，其富集倍数高达30倍。这一步骤程序的优点是能够浓缩血液成分中出现的天然白细胞和非典型白细胞。后者可能是导致各种计数方法所得结果缺乏互换性的原因。
