Li Z, Yu L, Zhang Y, Gao J, Zhang P, Wan B, Chen C, Zhao S
Institute of Genetics, School of Life Science, Fudan University, Shanghai, PR China.
Mol Biol Rep. 2001;28(2):91-101. doi: 10.1023/a:1017998029053.
Three novel cDNAs encoding putative protein phosphatase-1 inhibitory protein (PPP1R14A) were isolated from human, mouse and rat. The human, mouse and rat PPP1R14A proteins were all of 147 amino acids sharing about 90% sequence identity to porcine CPI17, an inhibitor of protein phosphatase-1 holoenzyme. The protein sequence 33RHARVTVK40, which is important for the inhibitory potency of porcine CPI17, was conserved in mammalian PPP1R14A. Northern blot analysis showed that human PPP1R14A was highly expressed as a 600 bp transcript in heart, prostate, testis, ovary, colon, small intestine and pancreas, lowly in brain, placenta, skeletal muscle and spleen and lung. The distribution of mouse and rat PPP1R14A was more specific and different from that of human PPP1R14A, abundant in lung, moderate/abundant in testis, moderate in brain and low in heart. In addition, we mapped human PPP1R14A to chromosome 19q13.13-q13.2 by radiation hybrid mapping, and determined that the human PPP1R14A gene spanned a 5.1-kb region and consisted of four exons and three introns.
从人、小鼠和大鼠中分离出了三种编码假定蛋白磷酸酶-1抑制蛋白（PPP1R14A）的新cDNA。人、小鼠和大鼠的PPP1R14A蛋白均由147个氨基酸组成，与猪CPI17（一种蛋白磷酸酶-1全酶抑制剂）的序列同一性约为90%。对猪CPI17抑制效力很重要的蛋白序列33RHARVTVK40在哺乳动物PPP1R14A中保守。Northern印迹分析表明，人PPP1R14A作为一个600bp的转录本在心脏、前列腺、睾丸、卵巢、结肠、小肠和胰腺中高表达，在脑、胎盘、骨骼肌、脾脏和肺中低表达。小鼠和大鼠PPP1R14A的分布更具特异性，与人PPP1R14A不同，在肺中丰富，在睾丸中中等/丰富，在脑中中等，在心脏中低表达。此外，我们通过辐射杂种定位将人PPP1R14A定位于染色体19q13.13-q13.2，并确定人PPP1R14A基因跨越5.1kb区域，由四个外显子和三个内含子组成。
