PGF2α对大鼠第一代黄体中20α-羟基类固醇脱氢酶的诱导作用。

Induction by PGF2a of 20alpha-hydroxysteroid dehydrogenase in first generation corpora lutea of the rat.

作者信息

Lamprecht S A, Herlitz H V, Ahrén K E

出版信息

Mol Cell Endocrinol. 1975 Oct;3(4):273-82. doi: 10.1016/0303-7207(75)90009-x.

PMID:1193294

Abstract

20alpha-Hydroxysteroid dehydrogenase (20alpha-OH-SDH) activity was determined in the first generation corpora lutea from prepubertal rats injected with 10 I.U. of pregnant mare's serum gonadotrophin (PMSG) on day 30. The enzyme was not detectable in 1-9-day-old corpora lutea but a significant activity was seen on day 10. Enzyme activity increased during day 11 and day 12. In vivo administration of prostaglandin F2a (PGF2a) induced the enzyme in rats with corpora lutea older than 3 days. When prolactin was given concurrently with PGF2a, the corpus luteum activity of 20alpha-OH-SDH was lower than when PGF2a was given alone. It is concluded that the present "corpus luteum model" is suitable for further analysis of the cellular mechanisms of the luteolytic effect of prostaglandins (PGs) as well as of the role of gonadotrophins in the luteolytic process.

摘要

在第30天给青春期前大鼠注射10国际单位孕马血清促性腺激素（PMSG）后，测定第一代黄体中20α-羟基类固醇脱氢酶（20α-OH-SDH）的活性。在1至9日龄的黄体中未检测到该酶，但在第10天观察到显著活性。酶活性在第11天和第12天增加。体内给予前列腺素F2α（PGF2α）可在黄体超过3天的大鼠中诱导该酶。当催乳素与PGF2α同时给予时，20α-OH-SDH的黄体活性低于单独给予PGF2α时。结论是，目前的“黄体模型”适用于进一步分析前列腺素（PGs）黄体溶解作用的细胞机制以及促性腺激素在黄体溶解过程中的作用。