Lahav M, Lamprecht S A, Amsterdam A, Lindner H R
Mol Cell Endocrinol. 1977 Feb;6(4-5):293-302. doi: 10.1016/0303-7207(77)90103-4.
20alpha-Hydroxysteroid dehydrogenase (20 alpha-SDH) activity increases in the cycling corpus luteum of the rat, beginning at 14.00 h on the day of diestrus, but remains low in corpora lutea of pregnancy throughout the first 19 days of gestation. When cells derived from 7-day-old corpora lutea of pregnant rats were cultured for 7 or 12 days, there was a spontaneous rise in 20 alpha-SDH activity from an initial value of 0.44 +/- 0.27 to 4.1 +/- 0.7 units/mg supernatant protein. Addition of LH (NIH-S-18; 2.0 mug/ml) or prostaglandin F2alpha (2.8 X 10(-5) M) to the medium from day 4 to the end of incubation period caused a slight but significant reduction in 20 alpha-SDH activity (20%, P less than 0.05). Supplementation of the medium with ovine prolactin (HIH-P-S11; 10.0 mug/ml) from the time of seeding or from the 2nd to 4th day of culture reduced the activity of 20 alpha-SDH measured on day 12 by 61% (P less than 0.001). This finding suggests that the suppression of 20 alpha-SDH by prolactin, hitherto demonstrated only in vivo results from a direct action of the hormone on the luteal cell.
20α-羟基类固醇脱氢酶（20α-SDH）活性在大鼠的周期性黄体中增加，从动情后期当天的14:00开始，但在妊娠的前19天整个妊娠期黄体中保持较低水平。当将来自妊娠大鼠7日龄黄体的细胞培养7天或12天时，20α-SDH活性从初始值0.44±0.27自发升高至4.1±0.7单位/毫克上清液蛋白。从第4天到培养期结束向培养基中添加促黄体生成素（NIH-S-18；2.0微克/毫升）或前列腺素F2α（2.8×10⁻⁵ M）导致20α-SDH活性略有但显著降低（20%，P＜0.05）。从接种时起或从培养的第2天到第4天向培养基中补充绵羊催乳素（HIH-P-S11；10.0微克/毫升）可使第12天测得的20α-SDH活性降低61%（P＜0.001）。这一发现表明，催乳素对20α-SDH的抑制作用，迄今仅在体内得到证实，是该激素对黄体细胞直接作用的结果。
