Berki Timea, Tavakoli Afshin, Nagy Károly Kalmár, Nagy Gergely, Németh Péter
Department of Immunology and Biotechnology, Faculty of Medicine, University of Pécs, Szigeti út 12, 7643 Pécs, Hungary.
Transpl Int. 2002 Mar;15(2-3):132-8. doi: 10.1007/s00147-002-0397-x. Epub 2002 Mar 9.
Previous studies have demonstrated that glucocorticoid resistance develops in some patients during glucocorticoid therapy due to the down-regulation of glucocorticoid receptor (GCR) expression. A new flow cytometric method has been used for monitoring the intracellular GCR level in peripheral blood mononuclear cells (PBMCs). GCR expression by different lymphocyte subpopulations (bearing surface CD4+ CD8+ or CD19+) in steroid-treated (ST) and non-treated (NT) renal transplant patients was also compared. High, decreasing-dose steroid treatment caused a reduced GCR expression in 50% of the renal transplant patients. Long-lasting, low-dose steroid therapy caused a fall in GCR level in only 11% of patients. CD4+ T lymphocytes showed both the lowest GCR level and its alteration due to steroid therapy, while CD8+ T cells and CD19+ B lymphocytes expressed higher GCR levels and were more sensitive to steroid treatment. Detection of GCR expression in lymphocytes may provide useful information about the changes of glucocorticoid sensitivity during the therapy.
先前的研究表明，在糖皮质激素治疗期间，一些患者会因糖皮质激素受体（GCR）表达下调而产生糖皮质激素抵抗。一种新的流式细胞术方法已被用于监测外周血单核细胞（PBMC）中的细胞内GCR水平。还比较了接受类固醇治疗（ST）和未接受治疗（NT）的肾移植患者中不同淋巴细胞亚群（表面带有CD4 +、CD8 +或CD19 +）的GCR表达。高剂量递减的类固醇治疗使50%的肾移植患者的GCR表达降低。长期低剂量类固醇治疗仅使11%的患者的GCR水平下降。CD4 + T淋巴细胞的GCR水平最低，且其因类固醇治疗而发生改变，而CD8 + T细胞和CD19 + B淋巴细胞表达较高的GCR水平，且对类固醇治疗更敏感。检测淋巴细胞中的GCR表达可能为治疗期间糖皮质激素敏感性的变化提供有用信息。
