Villegas-García J C, Santillán-Alarcón S
Laboratorio de Mastozoología, Departamento de Biología Animal, Centro de Investigaciones Biológicas, Universidad Autónoma del Estado de Morelos, Avenida Universidad No. 1 001, Colonia Chamilpa, Cuernavaca, Morelos 62210, México.
Rev Biol Trop. 2001 Jun;49(2):685-8.
Wild vectors and reservoir hosts of Trypanosoma cruzi were surveyed from February 1993 to June 1994 in Ticumán (18 degrees 46'N, 99 degrees 07'W), Mexico (Deciduous Tropical Forest). Direct faeces examination showed that 87% of Triatoma pallidipennis hosted the parasite; T. cruzi forms were present in cultures inoculated with faeces of fifty 67% triatomine bugs and thirty CD-1 strain mice (10 d old) inoculated (peritoneum) with faeces of positive insects T. cruzi amastigotes were found in heart 67%, kidneys 47%, liver 80%, lungs 50%, oesophagus 60%, skin 23%, spleen 73% and stomach 60%. T. cruzi was isolated by direct blood examination from seven 21% chiropterans and five 38% rodents and T. cruzi forms were present in cultures inoculated with blood of twenty-three 68% chiropterans and seven 54% rodents and T. cruzi amastigotes were seen in the kidneys of one 3% chiropterans and four 31% rodents and only in one Pteronotus parnellii mexicanus, organisms were seen in skin 2%. There was no association between organs and T. cruzi infection (p > 0.05).
1993年2月至1994年6月，在墨西哥蒂库曼（北纬18度46分，西经99度07分，落叶热带森林）对克氏锥虫的野生传播媒介和储存宿主进行了调查。直接粪便检查显示，87%的苍白锥蝽携带该寄生虫；用50只锥蝽的粪便接种培养物以及用30只10日龄CD - 1品系小鼠（经腹腔）接种阳性昆虫的粪便后，培养物中出现了克氏锥虫形态；在心脏中发现克氏锥虫无鞭毛体的占67%，肾脏中占47%，肝脏中占80%，肺中占50%，食道中占60%，皮肤中占23%，脾脏中占73%，胃中占60%。通过直接血液检查从7只（21%）翼手目动物和5只（38%）啮齿动物中分离出克氏锥虫，用23只（68%）翼手目动物和7只（54%）啮齿动物的血液接种培养物后，培养物中出现了克氏锥虫形态；在1只（3%）翼手目动物和4只（31%）啮齿动物的肾脏中发现了克氏锥虫无鞭毛体，仅在1只墨西哥帕氏叶鼻蝠的皮肤中发现了该生物体（占2%）。器官与克氏锥虫感染之间无关联（p>0.05）。
