利用磷酸结合蛋白的电位型磷酸盐传感系统。
Potentiometric phosphate-sensing system utilizing phosphate-binding protein.
作者信息
Kubo Izumi
机构信息
Department of Bioengineering, Faculty of Engineering, Soka University, Hachioji, Tokyo, Japan
出版信息
Anal Bioanal Chem. 2002 Jan;372(2):273-5. doi: 10.1007/s00216-001-1138-1. Epub 2001 Dec 15.
A phosphate-detection system has been developed which uses phosphate-binding protein (PBP) from Escherichia coli. PBP was immobilized on a sheet of nitrocellulose membrane by cross-linking and the membrane potential of the immobilized PBP was measured. The response time of the system to phosphate was 5 min. The response was selective to phosphate among other anions. Under optimum conditions 0.1-1.5 mmol L(-1) phosphate can be determined with this system.
已开发出一种利用大肠杆菌的磷酸盐结合蛋白（PBP）的磷酸盐检测系统。通过交联将PBP固定在硝酸纤维素膜片上，并测量固定化PBP的膜电位。该系统对磷酸盐的响应时间为5分钟。该响应在其他阴离子中对磷酸盐具有选择性。在最佳条件下，该系统可测定0.1 - 1.5 mmol L(-1)的磷酸盐。
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