Tellier Frédérique, Fritz René, Leroux Pierre, Carlin-Sinclair Abel, Cherton Jean-Claude
Unité de Phytopharmacie et Médiateurs Chimiques, Versailles, France.
J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Mar 25;769(1):35-46. doi: 10.1016/s1570-0232(01)00615-8.
The metabolism of cyano-oxime fungicide 1-(2-cyano-2-methoxyiminoacetyl)-3-ethylurea (cymoxanil) and analogs was studied on several strains of the fungus Botrytis cinerea owing to their difference in sensitivity towards cymoxanil. Chromatographic analysis of the unextracted culture medium was simpler and more accurate, particularly for ionizable metabolites because it avoids problems associated with extraction. Reversed-phase high-performance liquid chromatography was applied to compare the decrease of cymoxanil and analogs caused by different strains of B. cinerea, by periodic injections of incubated culture medium aliquots, directly on a C4 wide-pore column. Furthermore, a thin-layer chromatographic monitoring on C18 bonded silica gel with ion-pairing allowed the monitoring of the ionizable metabolites for substrates that were demonstrated to decompose most rapidly. These complementary analyses showed that the sensitivity of the highly sensitive strain towards cymoxanil was related to the disappearance of cyano-oximes studied from culture medium, namely to the ability of the strain B. cinerea to metabolize them.
由于几种灰葡萄孢菌菌株对霜脲氰的敏感性存在差异，因此研究了氰基肟类杀菌剂1-(2-氰基-2-甲氧基亚氨基乙酰基)-3-乙基脲（霜脲氰）及其类似物的代谢情况。对未提取的培养基进行色谱分析更简单、更准确，特别是对于可电离的代谢物，因为它避免了与提取相关的问题。采用反相高效液相色谱法，通过定期直接在C4宽孔柱上注入培养的培养基等分试样，比较不同灰葡萄孢菌菌株引起的霜脲氰及其类似物的减少情况。此外，在具有离子对的C18键合硅胶上进行薄层色谱监测，可以监测已证明分解最快的底物的可电离代谢物。这些补充分析表明，高敏感菌株对霜脲氰的敏感性与培养基中所研究的氰基肟的消失有关，即与灰葡萄孢菌菌株代谢它们的能力有关。
