Biagini P, Benkoel L, Dodero F, de Lamballerie X, Chamlian V, Nouhou H, Gerolami V, de Micco P, Chamlian A
Laboratoire de Virologie Moléculaire, Tropicale et Transfusionnelle, Faculté de Médecine, Marseille, France.
Cell Mol Biol (Noisy-le-grand). 2001;47 Online Pub:OL167-71.
The purpose of this study was to localize HCV RNA in formalin-fixed paraffin-embedded liver biopsies of 15 patients with chronic hepatitis C using in situ RT-PCR method. The results were compared to serum and tissue extract analysis of HCV RNA. HCV RNA was detected in 80% of the sera tested, in 40% of the corresponding hepatic tissue extract and in 60% of the tissue sections tested by in situ RT-PCR. Compared to the serum positive cases, 67% of the cases were positive with in situ RT-PCR and 41% were positive with tissue extract detection. 50% of the cases in situ RT-PCR positive were also positive with tissue extract detection. These results underlined the complementarity of the different methods of viral detection for the precise diagnosis of hepatitis C.
本研究的目的是使用原位逆转录聚合酶链反应（in situ RT-PCR）方法，对15例慢性丙型肝炎患者经福尔马林固定、石蜡包埋的肝活检组织中的丙型肝炎病毒（HCV）RNA进行定位。将结果与HCV RNA的血清及组织提取物分析结果进行比较。在80%检测的血清、40%相应的肝组织提取物以及60%经原位RT-PCR检测的组织切片中检测到HCV RNA。与血清阳性病例相比，67%的病例原位RT-PCR呈阳性，41%的病例组织提取物检测呈阳性。原位RT-PCR阳性的病例中有50%组织提取物检测也呈阳性。这些结果强调了不同病毒检测方法在丙型肝炎精确诊断中的互补性。
