Baldo B A, Uhlenbruck G
Immunology. 1975 Dec;29(6):1161-70.
A powerful natural agglutinin with haemagglutinating and precipitating properties has been found in the haemolymph from the elongate clam Tridacna maxima (Röding). The agglutinin shows anti-galactan properties and reacts with a variety of bacteria-, plant-, invertebrate- and vertebrate-derived galactans, glycopeptides and polysaccharides. Previous studies which showed that the agglutinin-combining sites were inhibited by 2-acetamido-2-deoxy-D-galactose and D-galactose also revealed some anomalies in the reactions of the agglutinin with different saccharide-containing macromolecules. In an attempt to resolve these anomalies, the present study further examines the precipitating and haemagglutinating properties of the agglutinin and illustrates a requirement of the agglutinin for Ca2+. The ability of the T. maxima agglutinin to precipitate with structures containing terminal beta-linked 2-acetamido-2-deoxy-D-galactopyranosyl or D-galactopyranosyl groups suggests that the Tridacna haemolymph and purified lectin will find considerable application in the study of many biologically important carbohydrates.
在大砗磲（Tridacna maxima，罗丁）的血淋巴中发现了一种具有强大血细胞凝集和沉淀特性的天然凝集素。该凝集素具有抗半乳聚糖特性，可与多种源自细菌、植物、无脊椎动物和脊椎动物的半乳聚糖、糖肽和多糖发生反应。先前的研究表明，2-乙酰氨基-2-脱氧-D-半乳糖和D-半乳糖可抑制凝集素结合位点，同时也揭示了该凝集素与不同含糖类大分子反应中的一些异常现象。为了解决这些异常问题，本研究进一步考察了该凝集素的沉淀和血细胞凝集特性，并阐明了该凝集素对Ca2+的需求。大砗磲凝集素与含有末端β-连接的2-乙酰氨基-2-脱氧-D-吡喃半乳糖基或D-吡喃半乳糖基结构发生沉淀反应的能力表明，砗磲血淋巴和纯化的凝集素在许多具有生物学重要性的碳水化合物研究中将有广泛应用。
