在原索动物皮海鞘的血淋巴中鉴定出两种碳水化合物特异性红细胞凝集素。
Identification of two carbohydrate-specific erythrocyte agglutinins in the haemolymph of the protochordate Botrylloides leachii.
作者信息
Schluter S F, Ey P L, Keough D R, Jenkin C R
出版信息
Immunology. 1981 Feb;42(2):241-50.
The haemolymph of the colonial ascidian Botrylloides leachii contains two haemagglutinins which have been termed HA-1 and HA-2 respectively. The HA-1 agglutinin is specific for guinea-pig erythrocytes, requires Ca2+ ions for its activity and has an apparent mol. wt. of about 200,000. It can be adsorbed by guinea-pig but not human, sheep, mouse or pigeon erythrocytes. The agglutination of guinea-pig cells is reversibly inhibited by various mono- and oligosaccharides, lactose and D-galactose being among the most effective of those tested. The HA-2 agglutinin is smaller (mol.wt. approximately 63,000) and agglutinates a variety of erythrocyte types, including those from the human, guinea-pig, mouse, sheep, pigeon and chicken. The agglutination of all these cells is inhibited by lactose. Data from sugar-inhibition and adsorption experiments indicate that the HA-2 agglutinins are homogeneous with respect to binding site specificity. Divalent cations are not required for activity.
群体海鞘Botrylloides leachii的血淋巴含有两种血凝素，分别称为HA - 1和HA - 2。HA - 1血凝素对豚鼠红细胞具有特异性，其活性需要Ca2 +离子，表观分子量约为200,000。它可以被豚鼠红细胞吸附，但不能被人、绵羊、小鼠或鸽子的红细胞吸附。豚鼠细胞的凝集反应可被各种单糖和寡糖可逆抑制，乳糖和D -半乳糖是测试中最有效的糖类。HA - 2血凝素较小（分子量约为63,000），能凝集多种红细胞类型，包括来自人、豚鼠、小鼠、绵羊、鸽子和鸡的红细胞。所有这些细胞的凝集反应都被乳糖抑制。糖抑制和吸附实验的数据表明，HA - 2血凝素在结合位点特异性方面是同质的。其活性不需要二价阳离子。
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