Tsuboi Yasuhiro, Kim Youngki, Giebink G Scott, Le Chap, Paparella Michael M, Chen Nengqian, Schachern Patricia A, Juhn Steven K, Lin Jizhen
Department of Otolaryngology, University of Minnesota Otitis Media Research Center, University of Minnesota School of Medicine, Minneapolis, USA.
Acta Otolaryngol. 2002 Mar;122(2):153-60. doi: 10.1080/00016480252814153.
Otitis media with mucoid effusion, characterized by mucous cell metaplasia in the middle ear cleft and thick fluid accumulation in the middle ear cavity, is a common otological disease that frequently affects young children. Multiple factors are involved in the development of this disease, especially middle ear infection and Eustachian tube dysfunction. In this study, in order to induce otitis media with effusion in rats, we introduced a three-step method, namely inoculation of Streptococcus pneumoniae at 10(7) colony-forming units (CFU)/ear or Haemophilus influenzae at 5 x 10(7) CFU/ear into the middle ear cavity twice at 2-week intervals, followed by Eustachian tube obstruction (ETO) for 4 and 8 weeks. Animals inoculated with phosphate-buffered saline (PBS) twice in the same manner followed by ETO served as controls. Middle ear effusion and mucosa were harvested for evaluation of carbohydrate concentrations and mucous cell density, respectively. We found that rats inoculated with S. pneumoniae twice, followed by ETO at 8 weeks, yielded the highest carbohydrate concentration in middle ear effusion and the highest goblet cell density in the middle ear cavity compared to the H. influenzae and PBS groups. It is tentatively concluded that inoculation of S. pneumoniae at 10(7) CFU/ear into the middle ear cavity of rats twice at 2-week intervals, followed by ETO for 8 weeks, is a promising animal model for otitis media with mucoid effusion which may be valuable for studying the human counterpart.
分泌性中耳炎，其特征为中耳裂内的黏液细胞化生以及中耳腔内的浓稠液体积聚，是一种常见的耳部疾病，常影响幼儿。该疾病的发生涉及多种因素，尤其是中耳感染和咽鼓管功能障碍。在本研究中，为了在大鼠中诱导分泌性中耳炎，我们引入了一种三步法，即每隔2周向中耳腔内接种10⁷菌落形成单位（CFU）/耳的肺炎链球菌或5×10⁷CFU/耳的流感嗜血杆菌两次，随后进行4周和8周的咽鼓管阻塞（ETO）。以相同方式两次接种磷酸盐缓冲盐水（PBS）并随后进行ETO的动物作为对照。分别收集中耳积液和黏膜以评估碳水化合物浓度和黏液细胞密度。我们发现，与流感嗜血杆菌组和PBS组相比，两次接种肺炎链球菌并在8周后进行ETO的大鼠中耳积液中的碳水化合物浓度最高，中耳腔内杯状细胞密度也最高。初步得出结论，每隔2周向大鼠中耳腔内接种10⁷CFU/耳的肺炎链球菌两次，随后进行8周的ETO，是一种有前景的分泌性中耳炎动物模型，可能对研究人类同类疾病有价值。
