• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

采用三步法诱导大鼠中耳黏液细胞化生:一种改进的黏液样渗出性中耳炎模型

Induction of mucous cell metaplasia in the middle ear of rats using a three-step method: an improved model for otitis media with mucoid effusion.

作者信息

Tsuboi Yasuhiro, Kim Youngki, Giebink G Scott, Le Chap, Paparella Michael M, Chen Nengqian, Schachern Patricia A, Juhn Steven K, Lin Jizhen

机构信息

Department of Otolaryngology, University of Minnesota Otitis Media Research Center, University of Minnesota School of Medicine, Minneapolis, USA.

出版信息

Acta Otolaryngol. 2002 Mar;122(2):153-60. doi: 10.1080/00016480252814153.

DOI:10.1080/00016480252814153
PMID:11936906
Abstract

Otitis media with mucoid effusion, characterized by mucous cell metaplasia in the middle ear cleft and thick fluid accumulation in the middle ear cavity, is a common otological disease that frequently affects young children. Multiple factors are involved in the development of this disease, especially middle ear infection and Eustachian tube dysfunction. In this study, in order to induce otitis media with effusion in rats, we introduced a three-step method, namely inoculation of Streptococcus pneumoniae at 10(7) colony-forming units (CFU)/ear or Haemophilus influenzae at 5 x 10(7) CFU/ear into the middle ear cavity twice at 2-week intervals, followed by Eustachian tube obstruction (ETO) for 4 and 8 weeks. Animals inoculated with phosphate-buffered saline (PBS) twice in the same manner followed by ETO served as controls. Middle ear effusion and mucosa were harvested for evaluation of carbohydrate concentrations and mucous cell density, respectively. We found that rats inoculated with S. pneumoniae twice, followed by ETO at 8 weeks, yielded the highest carbohydrate concentration in middle ear effusion and the highest goblet cell density in the middle ear cavity compared to the H. influenzae and PBS groups. It is tentatively concluded that inoculation of S. pneumoniae at 10(7) CFU/ear into the middle ear cavity of rats twice at 2-week intervals, followed by ETO for 8 weeks, is a promising animal model for otitis media with mucoid effusion which may be valuable for studying the human counterpart.

摘要

分泌性中耳炎,其特征为中耳裂内的黏液细胞化生以及中耳腔内的浓稠液体积聚,是一种常见的耳部疾病,常影响幼儿。该疾病的发生涉及多种因素,尤其是中耳感染和咽鼓管功能障碍。在本研究中,为了在大鼠中诱导分泌性中耳炎,我们引入了一种三步法,即每隔2周向中耳腔内接种10⁷菌落形成单位(CFU)/耳的肺炎链球菌或5×10⁷CFU/耳的流感嗜血杆菌两次,随后进行4周和8周的咽鼓管阻塞(ETO)。以相同方式两次接种磷酸盐缓冲盐水(PBS)并随后进行ETO的动物作为对照。分别收集中耳积液和黏膜以评估碳水化合物浓度和黏液细胞密度。我们发现,与流感嗜血杆菌组和PBS组相比,两次接种肺炎链球菌并在8周后进行ETO的大鼠中耳积液中的碳水化合物浓度最高,中耳腔内杯状细胞密度也最高。初步得出结论,每隔2周向大鼠中耳腔内接种10⁷CFU/耳的肺炎链球菌两次,随后进行8周的ETO,是一种有前景的分泌性中耳炎动物模型,可能对研究人类同类疾病有价值。

相似文献

1
Induction of mucous cell metaplasia in the middle ear of rats using a three-step method: an improved model for otitis media with mucoid effusion.采用三步法诱导大鼠中耳黏液细胞化生:一种改进的黏液样渗出性中耳炎模型
Acta Otolaryngol. 2002 Mar;122(2):153-60. doi: 10.1080/00016480252814153.
2
Induction of mucous cell metaplasia by tumor necrosis factor alpha in rat middle ear: the pathological basis for mucin hyperproduction in mucoid otitis media.肿瘤坏死因子α诱导大鼠中耳黏液细胞化生:分泌性中耳炎黏液过度产生的病理基础。
Ann Otol Rhinol Laryngol. 2002 May;111(5 Pt 1):415-22. doi: 10.1177/000348940211100506.
3
Increased secretory capacity of the middle ear mucosa after acute otitis media caused by Haemophilus influenzae type B.由B型流感嗜血杆菌引起的急性中耳炎后中耳黏膜分泌能力增强。
Otolaryngol Head Neck Surg. 1997 Sep;117(3 Pt 1):263-7. doi: 10.1016/s0194-5998(97)70184-0.
4
A rat model of otitis media with effusion caused by eustachian tube obstruction with and without Streptococcus pneumoniae infection: methods and disease course.伴有和不伴有肺炎链球菌感染的咽鼓管阻塞所致中耳积液大鼠模型:方法与病程
Otolaryngol Head Neck Surg. 2002 May;126(5):490-8. doi: 10.1067/mhn.2002.124935.
5
Analysis by cDNA microarrays of altered gene expression in middle ears of rats following pneumococcal infection.肺炎球菌感染后大鼠中耳基因表达改变的cDNA微阵列分析。
Int J Pediatr Otorhinolaryngol. 2002 Sep 24;65(3):203-11. doi: 10.1016/s0165-5876(02)00130-1.
6
Pattern changes of mucin gene expression with pneumococcal otitis media.肺炎球菌性中耳炎黏蛋白基因表达的模式变化
Int J Pediatr Otorhinolaryngol. 2001 Oct 19;61(1):23-30. doi: 10.1016/s0165-5876(01)00540-7.
7
Antigenic as well as nonantigenic stimuli induce similar middle ear responses in the rat.抗原性和非抗原性刺激在大鼠中引发相似的中耳反应。
Laryngoscope. 2003 Feb;113(2):322-7. doi: 10.1097/00005537-200302000-00023.
8
Middle ear secretory capacity after acute otitis media caused by Streptococcus pneumoniae, Moraxella catarrhalis, non-typeable or type B Haemophilus influenzae. A comparative analysis based on goblet cell density.肺炎链球菌、卡他莫拉菌、不可分型或B型流感嗜血杆菌引起的急性中耳炎后的中耳分泌能力。基于杯状细胞密度的比较分析。
Acta Otolaryngol Suppl. 2000;543:54-5.
9
Accumulation of Regulatory T Cells and Chronic Inflammation in the Middle Ear in a Mouse Model of Chronic Otitis Media with Effusion Induced by Combined Eustachian Tube Blockage and Nontypeable Haemophilus influenzae Infection.在咽鼓管阻塞联合非分型流感嗜血杆菌感染诱导的慢性分泌性中耳炎小鼠模型中,中耳调节性T细胞的积累与慢性炎症
Infect Immun. 2015 Nov 9;84(1):356-64. doi: 10.1128/IAI.01128-15. Print 2016 Jan.
10
Mucosal expression of ENaC and AQP in experimental otitis media induced by Eustachian tube obstruction.咽鼓管阻塞诱导的实验性中耳炎中ENaC和AQP的黏膜表达
Int J Pediatr Otorhinolaryngol. 2009 Nov;73(11):1589-93. doi: 10.1016/j.ijporl.2009.08.011. Epub 2009 Sep 3.

引用本文的文献

1
Safety and Efficacy of Intratympanic Alpha-Lipoic Acid Injection in a Mouse Model of Noise-Induced Hearing Loss.鼓室内注射α-硫辛酸在噪声性听力损失小鼠模型中的安全性和有效性
Antioxidants (Basel). 2022 Jul 22;11(8):1423. doi: 10.3390/antiox11081423.
2
The Role of the Notch Signal Pathway in Mucosal Cell Metaplasia in Mouse Acute Otitis Media.Notch 信号通路在小鼠急性中耳炎黏膜细胞化生中的作用。
Sci Rep. 2017 Jul 4;7(1):4588. doi: 10.1038/s41598-017-04639-z.
3
SuperSILAC Quantitative Proteome Profiling of Murine Middle Ear Epithelial Cell Remodeling with NTHi.
用嗜肺军团菌对小鼠中耳上皮细胞重塑进行超稳定同位素标记氨基酸定量蛋白质组分析
PLoS One. 2016 Feb 9;11(2):e0148612. doi: 10.1371/journal.pone.0148612. eCollection 2016.
4
Math1, retinoic acid, and TNF-α synergistically promote the differentiation of mucous cells in mouse middle ear epithelial cells in vitro.Math1、维甲酸和 TNF-α 协同促进体外小鼠中耳上皮细胞粘液细胞的分化。
Pediatr Res. 2013 Sep;74(3):259-65. doi: 10.1038/pr.2013.103. Epub 2013 Jun 19.
5
NTHi induction of Cxcl2 and middle ear mucosal metaplasia in mice.NTHi 诱导的 Cxcl2 和中耳黏膜化生在小鼠中的作用。
Laryngoscope. 2013 Nov;123(11):E66-71. doi: 10.1002/lary.24097. Epub 2013 Apr 1.
6
Mucin production and mucous cell metaplasia in otitis media.中耳炎中的黏蛋白产生与黏液细胞化生
Int J Otolaryngol. 2012;2012:745325. doi: 10.1155/2012/745325. Epub 2012 May 22.
7
The role of atoh1 in mucous cell metaplasia.Atoh1在黏液细胞化生中的作用。
Int J Otolaryngol. 2012;2012:438609. doi: 10.1155/2012/438609. Epub 2012 Feb 14.
8
Activation of the transforming growth factor beta pathway in bacterial otitis media.细菌性中耳炎中转化生长因子β信号通路的激活
Ann Otol Rhinol Laryngol. 2011 Mar;120(3):204-13. doi: 10.1177/000348941112000310.
9
Interleukin-10 is an essential modulator of mucoid metaplasia in a mouse otitis media model.白细胞介素-10是小鼠中耳炎模型中黏液样化生的重要调节因子。
Ann Otol Rhinol Laryngol. 2008 Aug;117(8):630-6. doi: 10.1177/000348940811700814.