葡萄球菌肠毒素B甲醛化类毒素的促有丝分裂活性
Mitogenicity of formalinized toxoids of staphylococcal enterotoxin B.
作者信息
Spero L, Leatherman D L, Adler W H
出版信息
Infect Immun. 1975 Nov;12(5):1018-20. doi: 10.1128/iai.12.5.1018-1020.1975.
Staphylococcal enterotoxin B is a potent mitogen for mouse and human lymphocytes. Mitogenic activity was retained after detoxification of the enterotoxin by formaldehyde at pH 5.0, 7.5, OR 9.5. The most active toxoid (pH 7.5) was separated into a monomeric, a dimeric, and a polymeric fraction (1 x 10(5) to 3 x 10(5) molecular weight) by gel filtration, and although each fraction demonstrated mitogenic activity, the polymeric fraction was clearly the most efficacious. These data show that mitogenicity of staphylococcal enterotoxin B does not depend on toxicity. This suggests that the mitogenic and toxic activities are effected by different sites on the molecule.
葡萄球菌肠毒素B是一种对小鼠和人类淋巴细胞有强效作用的促细胞分裂剂。在pH值为5.0、7.5或9.5的条件下，用甲醛对肠毒素进行解毒后，其促有丝分裂活性得以保留。活性最高的类毒素（pH 7.5）通过凝胶过滤被分离成单体、二聚体和聚合物部分（分子量为1×10⁵至3×10⁵），尽管每个部分都表现出促有丝分裂活性，但聚合物部分显然最为有效。这些数据表明，葡萄球菌肠毒素B的促有丝分裂性并不取决于毒性。这表明促有丝分裂活性和毒性活性是由分子上不同的位点所介导的。
Zotero 插件