Greaves M, Janossy G, Doenhoff M
J Exp Med. 1974 Jul 1;140(1):1-18. doi: 10.1084/jem.140.1.1.
Human lymphocytes from spleen and tonsils have been cultured with a variety of polyclonal mitogens. Cultures consisted of either unseparated T and B cells or alternatively purified T or B lymphocytes. The purity of the starting cell populations and the origin of activated lymphoblasts was analyzed with a panel of seven markers which discriminate between T and B cells. The selectivity of the lymphocyte responses was influenced by cell populations in a given culture, the mitogen used, and to a limited extent on culture conditions. Purified T lymphocytes from tonsil and spleen responded to phytohemagglutinin (PHA), pokeweed mitogen (PWM), and staphylococcal enterotoxin B (SEB). Purified B cells from spleen responded well to PWM, weakly to SEB and lipopolysaccharide, but not at all to PHA. Tonsil B cells responded weakly to PWM and SEB but not to PHA. Some B lymphocytes do respond to PHA in the presence of activated T cells. These results are discussed in relation to previously reported selective responses of human cells and parallel studies in animal species.
来自脾脏和扁桃体的人类淋巴细胞已用多种多克隆有丝分裂原进行培养。培养物由未分离的T细胞和B细胞组成，或者是纯化的T淋巴细胞或B淋巴细胞。用一组七种可区分T细胞和B细胞的标志物分析起始细胞群体的纯度以及活化淋巴母细胞的来源。淋巴细胞反应的选择性受给定培养物中的细胞群体、所用有丝分裂原以及在有限程度上受培养条件的影响。来自扁桃体和脾脏的纯化T淋巴细胞对植物血凝素（PHA）、商陆有丝分裂原（PWM）和葡萄球菌肠毒素B（SEB）有反应。来自脾脏的纯化B细胞对PWM反应良好，对SEB和脂多糖反应较弱，但对PHA完全无反应。扁桃体B细胞对PWM和SEB反应较弱，但对PHA无反应。一些B淋巴细胞在活化T细胞存在的情况下确实对PHA有反应。结合先前报道的人类细胞的选择性反应以及动物物种的平行研究对这些结果进行了讨论。
