Nedelec O, Bellagra N, Devisme L, Hober D, Wattré P, Dewilde A
Service de virologie, CHRU de Lille, bâtiment Paul-Boulanger 59037 Lille, France.
Ann Biol Clin (Paris). 2002 Mar-Apr;60(2):201-7.
A quantitative PCR assay (RS Elosa CMV, Lambdatech) was used to quantitate HCMV DNA in maternal amniotic fluid of 12 fetuses with congenital infection (group 1) and of 10 fetuses without congenital infection (group 2). HCMV detection was performed for both groups using culture and qualitative PCR. Histologic examinations of fetal tissues and placenta were carried out for 9 patients from group 1. The amniotic fluid viral loads were negative in all patients of group 2. In group 1, all viral loads were high (from 1.105 to > 107 cop/mL) and no difference was observed between symptomatic and asymptomatic foetuses. Further evaluation on larger samples is needed to define more precisely the pronostic value of HCMV DNA quantification in amniotic fluid.
采用定量聚合酶链反应检测法（RS Elosa CMV，Lambdatech公司）对12例先天性感染胎儿（第1组）和10例无先天性感染胎儿（第2组）的母羊水中人巨细胞病毒（HCMV）DNA进行定量分析。两组均采用培养法和定性聚合酶链反应检测HCMV。对第1组9例患者的胎儿组织和胎盘进行组织学检查。第2组所有患者的羊水病毒载量均为阴性。在第1组中，所有病毒载量都很高（从1.105到>107拷贝/毫升），有症状和无症状胎儿之间未观察到差异。需要对更多样本进行进一步评估，以更精确地确定羊水中HCMV DNA定量分析的预后价值。
