Lukácsi A, Taródi B, Endreffy E, Pál A, Pusztai R
Department of Medical Microbiology, University of Szeged, Szeged H-6720, Hungary.
J Med Virol. 2001 Nov;65(3):537-42.
On the basis of the sequence variation of the glycoprotein B (gB) gene, human cytomegalovirus (HCMV) can be classified into four gB genotypes. Genotyping of HCMV from congenital infections was carried out on the assumption that the envelope gB may influence the outcome of prenatal infection. Sixty-three pregnant women were included in the study: 40 pregnant women whose fetuses were strongly suspected of having viral infection, and 23 women with normal pregnancies, from whom amniotic fluid was taken for fetal karyotype assessment. The amniotic fluid, fetal blood, blood, and urine of the newborns were examined for HCMV DNA by a nested polymerase chain reaction, and the gB genotype was determined by restriction fragment length polymorphism. HCMV DNA was detected in 12 cases in which the fetuses were suspected of having a viral infection and in 3 of the normal pregnancies. All the HCMV DNA had identical genotype, gB1. These data clearly indicate the dominance of the gB1 genotype in congenital HCMV infections. The clinical outcome of these pregnancies, however, cannot be predicted on the basis of the involvement of this genotype.
基于糖蛋白B（gB）基因的序列变异，人巨细胞病毒（HCMV）可分为四种gB基因型。对先天性感染的HCMV进行基因分型是基于包膜gB可能影响产前感染结果这一假设。该研究纳入了63名孕妇：40名胎儿被高度怀疑有病毒感染的孕妇，以及23名正常妊娠的孕妇，后者的羊水被用于胎儿核型评估。采用巢式聚合酶链反应检测新生儿的羊水、胎儿血液、血液和尿液中的HCMV DNA，并通过限制性片段长度多态性确定gB基因型。在12例胎儿被怀疑有病毒感染的病例以及3例正常妊娠病例中检测到了HCMV DNA。所有HCMV DNA具有相同的基因型，即gB1。这些数据清楚地表明gB1基因型在先天性HCMV感染中占主导地位。然而，无法根据该基因型的情况预测这些妊娠的临床结局。
