[Construction of three transgenic vectors carrying the latent membrane protein gene of Epstein-Barr virus].

In order to produce transgenic mice carrying the latent membrane protein(LMP) gene of Epstein-Barr virus(EBV) and to study the oncogenic role of LMP gene in vivo, three different transgenic vectors carrying the LMP gene were constructed. In pBR322-MT-LMP vector, the promoter of LMP gene is the regulation region of mouse metallothionein-I gene. In pMci3-LMP shuttle vector, the promoter of LMP gene is the replication origin(oriP) of EBV. In pMV-LMP-c-myc retroviral vector, the long terminal repeat (LTR) of mouse sarcomavirus serves as the promoter of LMP gene. The characterization and usefulness of these three transgenic vectors are also discussed.