Takasu Takuma, Iles Alexander, Hasebe Kiyoshi
Division of Material Science, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Japan.
Anal Bioanal Chem. 2002 Feb;372(4):554-61. doi: 10.1007/s00216-001-1135-4. Epub 2001 Dec 21.
A simple, accurate and reproducible reversed-phase high-performance liquid chromatography (HPLC) method was developed for the separation and characterisation of alkylphenols (APs) and alkylphenol polyethoxylates (APEOs), using a C18 octadecyl silica (ODS) column. APs and each APEO oligomer were separated successfully within a reasonable time without gradient elution. An excellent resolution was obtained, even for mixtures of APs and low EO number APEOs, which are otherwise difficult to separate using conventional normal-phase HPLC methods. This method, combined with solid-phase extraction, was highly applicable for the simultaneous determination of alkylphenols and alkylphenol ethoxylates in real samples.
建立了一种简单、准确且可重复的反相高效液相色谱（HPLC）方法，用于分离和表征烷基酚（APs）和烷基酚聚乙氧基化物（APEOs），采用C18十八烷基硅胶（ODS）柱。APs和每种APEO低聚物在合理时间内成功分离，无需梯度洗脱。即使对于APs和低环氧乙烷（EO）数的APEOs混合物，也能获得出色的分离度，而使用传统正相HPLC方法则难以分离这些混合物。该方法与固相萃取相结合，非常适用于同时测定实际样品中的烷基酚和烷基酚乙氧基化物。
