West Robert B, Warnke Roger A, Natkunam Yasodha
Department of Pathology, Stanford University Medical Center, CA 94305-5302, USA.
Am J Clin Pathol. 2002 Apr;117(4):636-43. doi: 10.1309/W3QX-WJ1C-WG2K-225V.
We used a panel of paraffin antibodies to determine whether neoplastic and nonneoplastic lymphoid aggregates in the bone marrow can be distinguished reliably. Formalin-fixed, paraffin-embedded bone marrow core biopsy specimens with lymphoid aggregates were stained using primary antibodies directed against bcl-2, bcl-6, CD5, CD10, CD20, and CD23. We studied 61 cases (26 follicular lymphoma and 35 benign or atypical aggregates). We found that no single stain is sufficient for identification of neoplastic lymphoid aggregates. However, this distinction was made possible by using a panel of antibodies. Under the conditions we tested, the most useful antibodies were CD10, bcl-2, CD5, and CD20. Most benign or atypical aggregates do not express CD10 and CD23. In addition, nonneoplastic aggregates had a large population of T cells. bcl-2 was useful in an architectural context for distinguishing neoplastic aggregates. bcl-6 often was expressed in both neoplastic and nonneoplastic aggregates and, thus, poorly discriminated between these processes. We studied the expression of CD10 and bcl-6 in selected lymph nodes in some cases.
我们使用一组石蜡抗体来确定骨髓中的肿瘤性和非肿瘤性淋巴样聚集物是否能够被可靠地区分。使用针对bcl-2、bcl-6、CD5、CD10、CD20和CD23的一抗，对含有淋巴样聚集物的福尔马林固定、石蜡包埋的骨髓芯活检标本进行染色。我们研究了61例病例（26例滤泡性淋巴瘤和35例良性或非典型聚集物）。我们发现没有单一的染色足以鉴定肿瘤性淋巴样聚集物。然而，通过使用一组抗体可以实现这种区分。在我们测试的条件下，最有用的抗体是CD10、bcl-2、CD5和CD20。大多数良性或非典型聚集物不表达CD10和CD23。此外，非肿瘤性聚集物中有大量T细胞。bcl-2在区分肿瘤性聚集物的结构背景方面很有用。bcl-6在肿瘤性和非肿瘤性聚集物中通常都有表达，因此在区分这些过程方面效果不佳。在某些病例中，我们研究了所选淋巴结中CD10和bcl-6的表达情况。
