Cui Dan, Kawano Hiroo, Takahashi Mutsuo, Hoshii Yoshinobu, Setoguchi Mihoko, Gondo Toshikazu, Ishihara Tokuhiro
First Department of Pathology, Yamaguchi University School of Medicine, Ube, Japan.
Pathol Int. 2002 Jan;52(1):40-5. doi: 10.1046/j.1440-1827.2002.01309.x.
We herein report that experimental murine amyloid A (AA) deposition is accelerated by oral administration of semipurified amyloid fibrils extracted from different species. Three groups of mice were treated with semipurified murine AA amyloid fibrils, semipurified bovine AA amyloid fibrils or semipurified human light chain-derived (A(lambda)) amyloid fibrils for 10 days. After 3 weeks, each mouse was subjected to inflammatory stimulation by subcutaneous injection with a mixture of complete Freund's adjuvant supplemented with Mycobacterium butyricum. The mice were killed on the third day after the inflammatory stimulation, and the spleen, liver, kidney and gastrointestinal tract were examined for amyloid deposits. Amyloid deposits were detected in 14 out of 15 mice treated with murine AA amyloid fibrils, 12 out of 15 mice treated with bovine AA amyloid fibrils and 11 out of 15 mice treated with human A(lambda) amyloid fibrils. No amyloid deposits were detected in control mice receiving the inflammatory stimulant alone or in amyloid fibril-treated mice without inflammatory stimulation. Our results suggest that AA amyloid deposition is accelerated by oral administration of semipurified amyloid fibrils when there is a concurrent inflammatory stimulation.
我们在此报告，口服从不同物种提取的半纯化淀粉样纤维可加速实验性小鼠淀粉样蛋白A（AA）沉积。三组小鼠分别用半纯化的小鼠AA淀粉样纤维、半纯化的牛AA淀粉样纤维或半纯化的人轻链衍生（A(lambda)）淀粉样纤维处理10天。3周后，每只小鼠通过皮下注射补充丁酸分枝杆菌的完全弗氏佐剂混合物进行炎症刺激。在炎症刺激后第三天处死小鼠，并检查脾脏、肝脏、肾脏和胃肠道中的淀粉样沉积物。在用小鼠AA淀粉样纤维处理的15只小鼠中有14只检测到淀粉样沉积物，在用牛AA淀粉样纤维处理的15只小鼠中有12只检测到淀粉样沉积物，在用人类A(lambda)淀粉样纤维处理的15只小鼠中有11只检测到淀粉样沉积物。在仅接受炎症刺激的对照小鼠或未接受炎症刺激的淀粉样纤维处理小鼠中未检测到淀粉样沉积物。我们的结果表明，当同时存在炎症刺激时，口服半纯化淀粉样纤维可加速AA淀粉样沉积。
