Cui Dan, Kawano Hiroo, Hoshii Yoshinobu, Liu Ye, Ishihara Tokuhiro
First Department of Pathology, Yamaguchi University School of Medicine, Ube, Yamaguchi, Japan.
Amyloid. 2008 Jun;15(2):77-83. doi: 10.1080/13506120802005833.
Acceleration of amyloid deposition by administration of amyloid fibrils and transmissibility of disease have been reported for several types of amyloidosis. Reactive amyloidosis (AA) occurs in a wide variety of domestic animal species and is characterized by amyloid deposition mainly in spleen, liver, and kidneys. Because the visceral organs of domestic animals have traditionally been used in Asian cuisines, it is important to examine whether dietary ingestion of the organs themselves (rather than purified amyloid fibrils) accelerates AA amyloid deposition. Herein, we show that murine AA amyloidosis develops rapidly after intraperitoneal or oral administration of purified amyloid fibrils or homogenates of amyloid-laden bovine liver. The amyloidosis development in mice was dependent on the concentration of amyloid fibrils or amyloidotic liver homogenates. We found that experimental murine AA amyloidosis was accelerated by dietary ingestion of both purified amyloid fibrils and tissue homogenates that contain amyloid fibrils. We also investigated livers of beef cattle and food chickens to examine whether they contain amyloid-enhancing factor activity. By microscopic examination of hematoxylin and eosin- and Congo red-stained sections, no amyloid deposition was detected in these livers, and no effective activity for experimental induction of AA amyloidosis in mice was detected in homogenates of these livers.
对于几种类型的淀粉样变性，已报道通过给予淀粉样纤维可加速淀粉样沉积以及疾病的传播性。反应性淀粉样变性（AA）发生于多种家畜物种，其特征是淀粉样沉积主要在脾脏、肝脏和肾脏。由于家畜的内脏器官传统上用于亚洲菜肴，因此研究通过饮食摄入这些器官本身（而非纯化的淀粉样纤维）是否会加速AA淀粉样沉积很重要。在此，我们表明，在腹腔内或口服给予纯化的淀粉样纤维或富含淀粉样物质的牛肝脏匀浆后，小鼠AA淀粉样变性迅速发展。小鼠淀粉样变性的发展取决于淀粉样纤维或淀粉样变性肝脏匀浆的浓度。我们发现，通过饮食摄入纯化的淀粉样纤维和含有淀粉样纤维的组织匀浆均可加速实验性小鼠AA淀粉样变性。我们还研究了肉牛和食用鸡的肝脏，以检查它们是否含有淀粉样增强因子活性。通过苏木精-伊红染色和刚果红染色切片的显微镜检查，在这些肝脏中未检测到淀粉样沉积，并且在这些肝脏的匀浆中未检测到对小鼠实验性诱导AA淀粉样变性的有效活性。
