Wang Hongman, Deng Huacong, Liu Wanli
Department of Endocrinology, The First Affiliated Hospital of Chongqing University of Medical Sciences, Chongqing 400016, China.
Zhonghua Nei Ke Za Zhi. 2002 Mar;41(3):179-82.
To explore the effect of paraoxonase-1 (PON-1) and oxidized low density lipoprotein (ox-LDL) in diabetic nephropathy (DN).
Serum PON-1 activity was measured by using phenyl acetate as a substrate and the concentration of plasma ox-LDL was determined by ELISA in 91 cases of type 2 diabetes mellitus (DM). Simultaneously, serum NO, VWF and GMP 140 were also determined. The patients of DM were divided into three groups according to urine albumine excretion rate (UAER) (DM I: UAER < 30 mg/24 h; DM II: UAER 30 approximately 300 mg/24 h; DM III: UAER > 300 mg/24 h) and matched with normal controls (NC).
In the three groups of DM, PON-1 were markedly decreased and ox-LDL were obviously increased (P < 0.01). Both of their levels were different in the three groups of DM (P < 0.05). PON-1 were negatively correlated with ox-LDL (r = -0.467, P < 0.01). There were negative correlation between PON-1 and urine albumin (r = -0.697, P < 0.01), but ox-LDL was positively correlated with urine albumin (r = 0.747, P < 0.01). Serum nitrites (NO) level in DM I was significantly higher than that in NC, but the NO level in DM II and DM III was lower than that in DM I and NC. GMP 140 and VWF levels in all the 3 DM groups were higher than that in NC. PON-1 was positively correlated with NO (r(s) = 0.326, P < 0.01), and negatively correlated with VWF (r(s) = -0.365, P < 0.01). ox-LDL was negatively correlated with NO (r(s) = -0.196, P < 0.05), but positively correlated with VWF and GMP 140 (r(s) = 0.294, P < 0.05; r(s) = 0.669, P < 0.01 respectively). Logistic regression showed that ox-LDL was a dangerous factor for DN.
PON-1, ox-LDL, NO, endothelial and platelet function are connected with DN; they affect one another and influence the occurrence and development of DN together.
探讨对氧磷酶-1（PON-1）和氧化型低密度脂蛋白（ox-LDL）在糖尿病肾病（DN）中的作用。
采用以苯乙酸为底物的方法测定91例2型糖尿病（DM）患者血清PON-1活性，用酶联免疫吸附测定法（ELISA）检测血浆ox-LDL浓度。同时，还测定了血清一氧化氮（NO）、血管性血友病因子（VWF）和颗粒膜蛋白140（GMP 140）。根据尿白蛋白排泄率（UAER）将DM患者分为三组（DM I：UAER < 30 mg/24 h；DM II：UAER 30～300 mg/24 h；DM III：UAER > 300 mg/24 h），并与正常对照组（NC）进行匹配。
在三组DM患者中，PON-1明显降低，ox-LDL明显升高（P < 0.01）。它们在三组DM患者中的水平也有所不同（P < 0.05）。PON-1与ox-LDL呈负相关（r = -0.467，P < 0.01）。PON-1与尿白蛋白呈负相关（r = -0.697，P < 0.01），而ox-LDL与尿白蛋白呈正相关（r = 0.747，P < 0.01）。DM I组血清亚硝酸盐（NO）水平显著高于NC组，但DM II组和DM III组的NO水平低于DM I组和NC组。所有3组DM患者的GMP 140和VWF水平均高于NC组。PON-1与NO呈正相关（r(s) = 0.326，P < 0.01），与VWF呈负相关（r(s) = -0.365，P < 0.01）。ox-LDL与NO呈负相关（r(s) = -0.196，P < 0.05），但与VWF和GMP 140呈正相关（分别为r(s) = 0.294，P < 0.05；r(s) = 0.669，P < 0.01）。Logistic回归分析显示ox-LDL是DN的危险因素。
PON-1、ox-LDL、NO、内皮及血小板功能与DN相关；它们相互影响，共同影响DN的发生发展。
