低温下的显微荧光测定法
Microfluorometric measurement at low temperature.
作者信息
Tiffe H W
出版信息
Histochemistry. 1975 Sep 7;45(1):77-81. doi: 10.1007/BF00508054.
PMID:1194086
Abstract
A cooling chamber for microfluorometric measurement is described, which allows to cool under a microscope a biological sample till 77 K and to measure it with an objective of a numerical aperture 0.6. From first experiments with BAO (5)-stained Tradescantia pollen it can be concluded, that experiments in this range of temperature open new aspects regarding the interpretation of microfluorometric phenomena.
摘要
本文描述了一种用于微荧光测量的冷却室，它能在显微镜下将生物样品冷却至77K，并使用数值孔径为0.6的物镜对其进行测量。通过对用BAO(5)染色的紫露草花粉进行的初步实验可以得出结论，在这个温度范围内进行的实验为微荧光现象的解释开辟了新的视角。
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