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低温下的显微荧光测定法

Microfluorometric measurement at low temperature.

作者信息

Tiffe H W

出版信息

Histochemistry. 1975 Sep 7;45(1):77-81. doi: 10.1007/BF00508054.

DOI:10.1007/BF00508054
PMID:1194086
Abstract

A cooling chamber for microfluorometric measurement is described, which allows to cool under a microscope a biological sample till 77 K and to measure it with an objective of a numerical aperture 0.6. From first experiments with BAO (5)-stained Tradescantia pollen it can be concluded, that experiments in this range of temperature open new aspects regarding the interpretation of microfluorometric phenomena.

摘要

本文描述了一种用于微荧光测量的冷却室，它能在显微镜下将生物样品冷却至77K，并使用数值孔径为0.6的物镜对其进行测量。通过对用BAO(5)染色的紫露草花粉进行的初步实验可以得出结论，在这个温度范围内进行的实验为微荧光现象的解释开辟了新的视角。

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Microfluorometric measurement at low temperature.低温下的显微荧光测定法
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引用本文的文献

1
A microscope stage sample holder for microfluorometric measurements of biological specimen in the range of temperature between 3.5 to 300 K.一种用于在3.5至300K温度范围内对生物样本进行微荧光测量的显微镜载物台样本架。
Histochemistry. 1977 May 20;52(2):171-7. doi: 10.1007/BF00492293.
2
The acridine dyes: their purification, physiochemical, and cytochemical properties. II. Purification of acriflavine and proflavine and their cytochemical behaviour regarding machine-oriented evaluation.吖啶染料：其纯化、物理化学及细胞化学性质。II. 吖啶黄和原黄素的纯化及其在面向机器评估方面的细胞化学行为。
Histochemistry. 1977 Jul 18;53(1):63-77. doi: 10.1007/BF00511211.