一种用于在3.5至300K温度范围内对生物样本进行微荧光测量的显微镜载物台样本架。

A microscope stage sample holder for microfluorometric measurements of biological specimen in the range of temperature between 3.5 to 300 K.

作者信息

Tiffe H W

出版信息

Histochemistry. 1977 May 20;52(2):171-7. doi: 10.1007/BF00492293.

Abstract

A cryostat was constructed, which permits measurements of the intracellular fluorescence within a range of temperature between 3.5 to 300 K. The cooling unit operates in accordance with the "Continuous Flow Principle" and allows the application of objectives up to a numerical aperture of 0.6. It results from measurement of BAO Feulgen stained nuclear DNA, that a decrease of fluorescence intensity is caused by two different mechanisms: (1) There is a highly temperature dependent effect, originating from phenomena of solid physics, and (2) a second effect, which is almost temperature independent, and can be explained as a photochemical reaction.

摘要

构建了一台低温恒温器，它能够在3.5至300 K的温度范围内测量细胞内荧光。冷却单元按照“连续流动原理”运行，并允许使用数值孔径高达0.6的物镜。通过对BAO Feulgen染色的核DNA进行测量发现，荧光强度的降低是由两种不同机制引起的：（1）存在一种高度依赖温度的效应，源于固体物理现象；（2）第二种效应几乎与温度无关，可解释为光化学反应。