Schneyer L H, Flatland R F
J Appl Physiol. 1975 Oct;39(4):519-22. doi: 10.1152/jappl.1975.39.4.519.
Cannulation of salivary gland main excretory duct at its oral opening is routinely used for collecting fluid, in situ, from the luminally perfused duct, or saliva from the stimulated gland. For perfusion of the main excretory duct, in situ, or for saliva collection, rat submaxillary gland is often the organ of choice, since electrolyte transport occurs at high rates both in the whole gland and in the main excretory duct. Recently, it has been reported that there is a pouchlike dilatation of the main excretory duct at its oral end, and that this dilatation may serve as a fluid reservoir. Because of possible effects of such a reservoir on measurements of electrolyte transport by the whole gland or the main duct segment, the size and form of the reservoir have now been examined. For this, techniques of histology, radiography, and microcatherization were employed. It was found that, while the functional volume of the reservoir exceeds that of the main duct proper, the time needed for displacement of reservoir fluid by perfusate or saliva would probably be only on the order of 1-3 min at higher rates of saliva or perfusate flow. Therefore, if adequate allowance is made for equilibration time, collection of saliva or luminal perfusate by oral cannula seems justified.
在唾液腺主排泄管的开口处进行插管，通常用于原位收集来自管腔灌注导管的液体或受刺激腺体分泌的唾液。由于整个腺体和主排泄管中电解质转运速率都很高，大鼠下颌下腺常被选作主排泄管灌注原位或唾液收集的器官。最近有报道称，主排泄管的开口端存在袋状扩张，且这种扩张可能起到液体储存库的作用。鉴于这样一个储存库可能会对整个腺体或主排泄管段的电解质转运测量产生影响，现在对储存库的大小和形态进行了研究。为此，采用了组织学、放射成像和微导管插入技术。结果发现，虽然储存库的功能容积超过了主排泄管本身，但在较高的唾液或灌注液流速下，灌注液或唾液置换储存库液体所需的时间可能仅为1 - 3分钟左右。因此，如果对平衡时间给予足够的考虑，通过口腔插管收集唾液或管腔灌注液似乎是合理的。
