Drochioiu G
Chemistry Faculty, Al. I. Cuza University of Iasi 11, Carol I, Iasi-6600, Romania.
Anal Bioanal Chem. 2002 Mar;372(5-6):744-7. doi: 10.1007/s00216-002-1238-6. Epub 2002 Feb 16.
A novel reaction of cyanide with 2,2-dihydroxy-1,3-indanedione in the presence of sodium carbonate is described. It is highly selective and sensitive, and suitable for the determination of hydrogen cyanide in the environment and free cyanide ions in water, blood, urine, serum, etc. As little as 1.25x10(-7) mol x L(-1) CN(-) (3.25x10(-9) g x mL(-1) cyanide) can be determined by use of this reaction. The color system obeys Beer's law in the range 10 ng x mL(-1) to 1.0 microg x mL(-1) at 510 nm. The molar absorptivity was 8.0x10(4) L x mol(-1) x cm(-1) for a solution of concentration 0.2 microg x mL(-1). All other important analytical properties of the reaction have been studied. It is proposed that the purple color produced under these reaction conditions is that of 2-cyano-1,2,3-trihydroxy-2 H indene.
描述了在碳酸钠存在下氰化物与2,2 - 二羟基 - 1,3 - 茚二酮的一种新型反应。该反应具有高选择性和高灵敏度，适用于环境中氰化氢以及水、血液、尿液、血清等中游离氰离子的测定。利用此反应可测定低至1.25×10⁻⁷ mol·L⁻¹的CN⁻（3.25×10⁻⁹ g·mL⁻¹氰化物）。该显色体系在510 nm波长下，10 ng·mL⁻¹至1.0 μg·mL⁻¹范围内符合比尔定律。对于浓度为0.2 μg·mL⁻¹的溶液，摩尔吸光系数为8.0×10⁴ L·mol⁻¹·cm⁻¹。已研究了该反应所有其他重要的分析特性。有人提出在这些反应条件下产生的紫色是2 - 氰基 - 1,2,3 - 三羟基 - 2H - 茚的颜色。
