[喉癌中谷氨酰胺转移酶3基因杂合性缺失及表达的研究]
[Study on the loss of heterozygosity and expression of transglutaminase 3 gene in laryngeal carcinoma].
作者信息
He Guang, Zhao Zhen, Fu Weineng, Sun Xinghe, Xu Zhenming, Sun Kailai
机构信息
Department of Medical Genetics, China Medical University, Shenyang, Liaoning 110001 P. R. China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2002 Apr;19(2):120-3.
OBJECTIVE
To investigate the role of transglutaminase 3 (TGM3) gene in laryngeal carcinogenesis.
METHODS
The authors detected the deletion indirectly through loss of heterozygosity (LOH) analysis at DNA level using 4 STR primers within and near TGM3 gene in 72 cases, and detected the differential expression of TGM3 gene in 8 cases of paired normal and cancerous tissue of laryngeal carcinoma by Northern blot.
RESULTS
LOH was found existing in all of the microsatellite loci, and the LOH frequencies were 25.76%, 20.00%, 38.10% and 18.75% at D20S17, D20S607, D20S99 and D20S841 respectively; LOH concerning at least one polymorphism locus accounted for 61.11%. No correlation of clinical stage, lymph node metastasis and differentiation with the LOH of TGM3 gene was observed, P>0.05. TGM3 gene expressed significantly higher in normal tissues than in paired cancerous tissues.
CONCLUSION
TGM3 gene might play an important role in laryngeal carcinogenesis and further researches will be needed to clarify the possible mechanisms.
目的
探讨转谷氨酰胺酶3（TGM3）基因在喉癌发生中的作用。
方法
作者采用位于TGM3基因内部及附近的4个STR引物，通过DNA水平的杂合性缺失（LOH）分析间接检测72例患者的缺失情况，并采用Northern印迹法检测8例喉癌配对正常组织和癌组织中TGM3基因的差异表达。
结果
所有微卫星位点均存在LOH，D20S17、D20S607、D20S99和D20S841位点的LOH频率分别为25.76%、20.00%、38.10%和18.75%；至少一个多态性位点的LOH占61.11%。未观察到TGM3基因的LOH与临床分期、淋巴结转移及分化之间存在相关性，P>0.05。TGM3基因在正常组织中的表达明显高于配对癌组织。
结论
TGM3基因可能在喉癌发生中起重要作用，尚需进一步研究以阐明其可能机制。
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