Punt Peter J, van Biezen Nick, Conesa Ana, Albers Alwin, Mangnus Jeroen, van den Hondel Cees
TNO Nutrition and Food Research Institute, Dept of Applied Microbiology and Gene Technology, P.O. Box 360, 3700 AJ, Zeist, The Netherlands.
Trends Biotechnol. 2002 May;20(5):200-6. doi: 10.1016/s0167-7799(02)01933-9.
Filamentous fungi have been used as sources of metabolites and enzymes for centuries. For about two decades, molecular genetic tools have enabled us to use these organisms to express extra copies of both endogenous and exogenous genes. This review of current practice reveals that molecular tools have enabled several new developments. But it has been process development that has driven the final breakthrough to achieving commercially relevant quantities of protein. Recent research into gene expression in filamentous fungi has explored their wealth of genetic diversity with a view to exploiting them as expression hosts and as a source of new genes. Inevitably, the progress in the 'genomics' technology will further develop high-throughput technologies for these organisms.
几个世纪以来，丝状真菌一直被用作代谢产物和酶的来源。在大约二十年的时间里，分子遗传学工具使我们能够利用这些生物体来表达内源性和外源性基因的额外拷贝。对当前实践的这一综述表明，分子工具带来了几项新进展。但正是工艺开发推动了在实现具有商业相关性的蛋白质产量方面的最终突破。最近对丝状真菌基因表达的研究探索了它们丰富的遗传多样性，以期将它们用作表达宿主和新基因的来源。不可避免地，“基因组学”技术的进步将进一步为这些生物体开发高通量技术。
