Ignatova E A, Nagornaia S S, Sudenko V I, Podgorskiĭ V S
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv.
Mikrobiol Z. 2002 Jan-Feb;64(1):20-6.
Life cycles of Candida utilis (Henneberg) Lodder et Kreger-van Rij imperfect yeast (13 strains) used in industry were the study subject. When the strains were mated, we detected one of the stages of sexual process--conjugation of cells of the opposite mating type. Most of the studied cultures conjugated on the 2d-3d day. No ascospores were formed. Haploidy and heterothallism of the studied C. utilis strains were confirmed by hybridization of auxotrophic mutants. Based on PCR assay results, the yeasts are demonstrated to belong to the ascospore perfect yeast species of Pichia jadinii (A. et R. Sartory. We ill et Meyer, Wickerham) Kurtzman.
以工业上使用的产朊假丝酵母（亨内贝格）洛德和克雷格 - 范·里伊不完全酵母（13个菌株）的生命周期为研究对象。当这些菌株进行交配时，我们检测到了有性过程的一个阶段——不同交配型细胞的接合。大多数被研究的培养物在第2至3天进行了接合。未形成子囊孢子。通过营养缺陷型突变体的杂交证实了所研究的产朊假丝酵母菌株的单倍体状态和异宗配合特性。基于聚合酶链反应（PCR）检测结果，这些酵母被证明属于雅丁毕赤酵母（A. 及R. 萨托里、韦尔、迈耶、威克汉姆）库尔茨曼的子囊孢子完全酵母物种。
