Rodrigues Maria Rita, Rodriguez Dunia, Russo Momtchilo, Campa Ana
Departamento de Análises Clínicas e Toxicológicas, Universidade de São Paulo, São Paulo, SP, CEP 05508-900, Brazil.
Biochem Biophys Res Commun. 2002 Apr 12;292(4):869-73. doi: 10.1006/bbrc.2002.6724.
Macrophages recovered from the peritoneum of mice, 48 h after concanavalin A administration, are primed and have a higher content of myeloperoxidase (MPO) than resident cells. The increase in MPO content is accompanied by an increased capability of macrophages generate hypochlorous acid and increased peroxidase activity. Contrary to the common sense, neutrophils is not the source of the MPO activity found in primed macrophages since macrophages recovered from mice treated with antigranulocyte antibody preserve the peroxidase activity. Given the broad spectrum of action of MPO, the preservation of MPO in primed macrophages might play a special role in the killing of pathogens and inflammation.
在注射伴刀豆球蛋白A 48小时后从小鼠腹膜中回收的巨噬细胞处于预激活状态，且与驻留细胞相比，其髓过氧化物酶（MPO）含量更高。MPO含量的增加伴随着巨噬细胞产生次氯酸的能力增强以及过氧化物酶活性增加。与常识相反，中性粒细胞并非预激活巨噬细胞中MPO活性的来源，因为从用抗粒细胞抗体处理的小鼠中回收的巨噬细胞保留了过氧化物酶活性。鉴于MPO的广泛作用谱，预激活巨噬细胞中MPO的保留可能在病原体杀伤和炎症中发挥特殊作用。
