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作者信息
Kopperschläger G, Diezel W, Bierwagen B, Hofmann E
机构信息
Physiologisch-chemisches Institut der Karl-Marx-Universität, Leipzig, DDR
出版信息
FEBS Lett. 1969 Nov 12;5(3):221-224. doi: 10.1016/0014-5793(69)80337-6.
A new method for molecular weight determination using polyacrylamidegel electrophoresis in a linear gel-concentration-gradient (3-20 degrees ) is described. Plotting the log of molecular weights of several standard proteins against distance of migration or against 3 log of rate of n-dgration reveal linear relationships in ranges of 50.000 - 200.000 or 100.000 - 400.000 Daltons respectively. On this basis, a simple method for molecular weight determination of proteins (accuracy +/- 5 degrees ) has been devised. The method can also be applied for an individual protein in protein mixtures using specific staining procedures.
本文描述了一种利用线性凝胶浓度梯度（3 - 20度）聚丙烯酰胺凝胶电泳测定分子量的新方法。绘制几种标准蛋白质的分子量对数与迁移距离或与迁移率的3倍对数的关系图，分别在50,000 - 200,000或100,000 - 400,000道尔顿范围内呈现线性关系。在此基础上，设计了一种简单的蛋白质分子量测定方法（准确度±5度）。该方法也可通过特定染色程序应用于蛋白质混合物中的单个蛋白质。
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