固体支持的镜像糖的合成：一种选择细胞表面碳水化合物受体的新方法。

Synthesis of solid-supported mirror-image sugars: a novel method for selecting receptors for cellular-surface carbohydrates.

作者信息

Kozlov I A, Mao S, Xu Y, Huang X, Lee L, Sears P S, Gao C, Coyle A R, Janda K D, Wong C H

机构信息

Department of Chemistry, The Skaggs Institute for Chemical Biology, La Jolla, CA 92037, USA.

出版信息

Chembiochem. 2001 Oct 1;2(10):741-6. doi: 10.1002/1439-7633(20011001)2:10<741::AID-CBIC741>3.0.CO;2-B.

DOI:10.1002/1439-7633(20011001)2:10<741::AID-CBIC741>3.0.CO;2-B

PMID:11948856

Abstract

We introduced a novel method, through mirror-image phage display, for the identification of high-affinity D-peptides to target specific cell-surface carbohydrates. Both 3-deoxy-alpha-L-manno-2-octulosonic acid (L-KDO) and L-sialic acid and an L-sialo-disaccharide have been synthesized and attached to a solid support for selection of high-affinity peptide binders displayed on phages. Our initial studies in this effort produce single-chain Fab sequences and dodecapeptides that bind to sialic acid and KDO with nanomolar and high micromolar affinity.

摘要

我们引入了一种通过镜像噬菌体展示来鉴定与特定细胞表面碳水化合物结合的高亲和力D-肽的新方法。3-脱氧-α-L-甘露糖-2-辛酮酸（L-KDO）、L-唾液酸和一种L-唾液酸二糖均已合成，并连接到固相载体上，用于筛选噬菌体展示的高亲和力肽结合物。我们在这项工作中的初步研究产生了以纳摩尔和高微摩尔亲和力与唾液酸和KDO结合的单链Fab序列和十二肽。