Nakajima Takeshi, Nakajima Emi, Fukiage Chiho, Azuma Mitsuyoshi, Shearer Thomas R
Senju Laboratory of Ocular Sciences, Oregon Health & Science University, West Campus, 505 N.W. 185th Avenue, Beaverton, OR 97006, U.S.A.
Exp Eye Res. 2002 Feb;74(2):231-6. doi: 10.1006/exer.2001.1131.
The mechanism causing loss of integrity of lens epithelial cells induced by an overdose of sodium selenite remains to be elucidated. The aim of the present experiment was to search for changes in gene expression in epithelial cells of lenses from rats developing selenite cataract. One day after injection of selenite into 12 day old rats, gene expression in lens epithelial cells was analysed using a commercial DNA array (Atlas Rat 1.2 Array). Changes were confirmed by RT-PCR. Of 1176 genes assayed by hybridization, 91 genes showed differences in expression between normal and selenite lenses. The three genes showing the greatest changes were: cytochrome c oxidase subunit I (COX-I, decrease), gastric inhibitory polypeptide (GIP, decrease), and early growth response protein-1 (EGR-1, increase). Both COX-I and EGR-1 have been reported to be involved with apoptosis. These results suggest that changes in COX-I and EGR-1 expression in lens epithelial cells might play important roles in apoptosis and altered metabolism leading to selenite cataract.
亚硒酸钠过量诱导晶状体上皮细胞完整性丧失的机制尚待阐明。本实验的目的是寻找患亚硒酸盐白内障大鼠晶状体上皮细胞基因表达的变化。给12日龄大鼠注射亚硒酸钠1天后，使用商业DNA阵列（Atlas Rat 1.2 Array）分析晶状体上皮细胞中的基因表达。通过逆转录聚合酶链反应（RT-PCR）确认变化。在通过杂交检测的1176个基因中，91个基因在正常晶状体和亚硒酸盐晶状体之间表现出表达差异。变化最大的三个基因是：细胞色素c氧化酶亚基I（COX-I，降低）、胃抑制多肽（GIP，降低）和早期生长反应蛋白-1（EGR-1，升高）。据报道，COX-I和EGR-1都与细胞凋亡有关。这些结果表明，晶状体上皮细胞中COX-I和EGR-1表达的变化可能在导致亚硒酸盐白内障的细胞凋亡和代谢改变中起重要作用。
