Laiko Victor V, Taranenko Nelli I, Berkout Vadym D, Yakshin Mikhail A, Prasad Coorg R, Lee H Sang, Doroshenko Vladimir M
MassTech Inc, Burtonsville, Maryland 20866, USA.
J Am Soc Mass Spectrom. 2002 Apr;13(4):354-61. doi: 10.1016/s1044-0305(02)00341-0.
A new atmospheric pressure (AP) infrared (IR) matrix-assisted laser desorption/ionization (MALDI) ion source was developed and interfaced with a Thermo Finnigan LCQ ion trap mass spectrometer. The source utilized a miniature all-solid-state optical parametric oscillator (OPO)-based IR laser system tunable in the lambda = 1.5-4 microm spectral range and a nitrogen ultraviolet (UV) laser (lambda = 337 nm) for use in comparative studies. The system demonstrated comparable performance at 3 microm and 337 nm wavelengths if UV matrices were used. However, AP IR-MALDI using a 3 microm wavelength showed good performance with a much broader choice of matrices including glycerol and liquid water. AP IR-MALDI mass spectra of peptides in the mass range up to 2000 Da were obtained directly from aqueous solutions at atmospheric conditions for the first time. A potential use of the new AP IR-MALDI ion source includes direct MS analysis of biological cells and tissues in a normal atmospheric environment as well as on-line coupling of mass spectrometers with liquid separation techniques.
一种新型的大气压（AP）红外（IR）基质辅助激光解吸/电离（MALDI）离子源被研发出来，并与一台赛默飞世尔（Thermo Finnigan）LCQ离子阱质谱仪相连接。该离子源采用了基于微型全固态光学参量振荡器（OPO）的红外激光系统，其波长可在λ = 1.5 - 4微米光谱范围内调谐，还配备了一台氮紫外（UV）激光（λ = 337纳米）用于对比研究。如果使用紫外基质，该系统在3微米和337纳米波长下表现出相当的性能。然而，使用3微米波长的大气压红外MALDI在包括甘油和液态水在内的更广泛的基质选择下展现出良好的性能。首次在大气条件下直接从水溶液中获得了质量范围高达2000道尔顿的肽段的大气压红外MALDI质谱。这种新型大气压红外MALDI离子源的一个潜在用途包括在正常大气环境中对生物细胞和组织进行直接质谱分析，以及将质谱仪与液相分离技术进行在线联用。
