通过聚合酶链反应早期诊断带状疱疹。
Early diagnosis of herpes zoster by polymerase chain reaction.
作者信息
Lilie H M, Wassilew S W, Wolff M H
机构信息
Dermatology Department, Klinikum Krefeld, Germany.
出版信息
J Eur Acad Dermatol Venereol. 2002 Jan;16(1):53-7. doi: 10.1046/j.0926-9959.2001.00351.x.
BACKGROUND
Herpes zoster is a common disease caused by the varicella-zoster virus. The use of virostatic agents as early as possible is necessary in shortening zoster-associated pain.
OBJECTIVES
Rapid diagnosis is necessary for the optimal efficacy of antiviral therapy. The diagnosis in the early stage of infection is often difficult.
METHODS
In the present study skin biopsies of patients with herpes zoster and unclear skin changes were analysed by detecting viral DNA using the polymerase chain reaction (PCR) in order to amplify open reading frames (ORF) 14, 29 and 63.
RESULTS
Varicella-zoster virus DNA could be detected with PCR of all three ORF not only from blisters but also from erythematous skin.
CONCLUSIONS
PCR is the method of choice for the viral diagnosis in herpes zoster before blister eruption.
背景
带状疱疹是由水痘-带状疱疹病毒引起的常见疾病。尽早使用抗病毒药物对于缩短带状疱疹相关疼痛是必要的。
目的
快速诊断对于抗病毒治疗的最佳疗效至关重要。感染早期的诊断通常很困难。
方法
在本研究中，对带状疱疹患者且皮肤变化不明确的患者进行皮肤活检，通过聚合酶链反应（PCR）检测病毒DNA，以扩增开放阅读框（ORF）14、29和63。
结果
通过PCR不仅可以从水疱中，还可以从红斑皮肤中检测到所有三个ORF的水痘-带状疱疹病毒DNA。
结论
PCR是水疱出现前带状疱疹病毒诊断的首选方法。
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